Madecassoside

製品コードS3843 バッチS384301

印刷

化学情報

Chemical Structure Synonyms Asiaticoside A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C48H78O20
分子量 975.12 CAS No. 34540-22-2
Solubility (25°C)* 体外 DMSO 100 mg/mL (102.55 mM)
Water 100 mg/mL (102.55 mM)
Ethanol 100 mg/mL (102.55 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Madecassoside (Asiaticoside A) is the main active triterpene constituent of Centella asiatica herbs, a traditional Chinese medicine for wound healing and scar management.
in vitro Madecassoside (10, 30, 100 μmol/L) could reverse morphological changes, elevate cell viability, increase glutathione levels, and decrease lactate dehydrogenase andmalondialdehyde levels caused by H2O2 in a concentration-dependent manner. It attenuates apoptosis, preventing the activation of caspase-3 and the loss of mitochondria membrane potential, as well as the phosphorylation of p38 mitogen activated protein kinase (MAPK) in HUVECs. Hence, Madecassoside could protect HUVECs against lipid peroxidation and apoptosis caused by H2O2[1].
in vivo The pharmacokinetic study of madecassoside in rats indicates that the maximum plasma concentration is 303.75 ± 28.53 ng/mL after single oral administration (100 mg/kg). Madecassoside, administered orally for 20 days at doses of 6, 12, and 24 mg/kg, effectively facilitates burn wound healing in mice. It could reduce the burn wound area, leading to a better healing pattern with almost complete wound closure in mice[1]. Madecassoside has potent anti-pulmonary fibrosis (PF) effects when administered p.o., despite having extremely low oral bioavailability. The potent anti-PF effects induced by p.o. madecassoside in mice are not mediated by its metabolites or itself after absorption into blood. i.p. madecassoside has no anti-PF effect. Madecassoside increases the activity of PPAR-γ, which subsequently increases HGF expression in colonic epithelial cells. HGF then enters into the circulation and lung tissue to exert an anti-PF effect[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 human umbilical vein endothelial cells (HUVECs)
濃度 10, 30, 100 μmol/L
反応時間 8 h
実験の流れ

HUVECs are harvested using 0.25% trypsin solution and inoculated in 96-well plates at a density of 1 × 104 cells/well. Cells are treated with H2O2 (500 μmol/L) and/or a variety of concentrations of madecassoside (10, 30, 100 μmol/L). The culture plate is then placed in 5% (v/v) CO2 at 37◦C for 8 h. At the end of incubation, 20 μL of MTT (5 mg/mL) is added to each well and the cells are incubated for another 4 h at 37◦C with 5% CO2. The supernatants are then discarded, and 200 μL of DMSO is added to each well. The absorbance is read at 540 nm and cell viability is expressed as percentage of untreated cells.
動物実験 動物モデル Female ICR mice, 6-8 weeks-old and weighing 22-26 g
投薬量 40 mg/kg
投与方法 p.o. or i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Importance of telomere shortening in the pathogenesis of ulcerative colitis: A new treatment from the aspect of telomeres in intestinal epithelial cells [ J Crohns Colitis, 2021, jjab115] PubMed: 34180971

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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