MCC950

製品コードS8930 バッチS893003

印刷

化学情報

Synonyms

CP-456773, CRID3

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₀H₂₄N₂O₅S

分子量

404.48

CAS No.

210826-40-7

Solubility (25°C)*

体外

DMSO

80 mg/mL (197.78 mM)

Ethanol

5 mg/mL (12.36 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	4.050mg/ml (10.01mM)	Taking the 1 mL working solution as an example, add 50 μL of 81 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.350mg/ml (0.87mM)	Taking the 1 mL working solution as an example, add 50 μL of 7 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	MCC950 (CP-456773, CRID3) is a potent and selective inhibitor of NLRP3 (NOD-like receptor (NLR) family, pyrin domain-containing protein 3) with IC50 of 7.5 nM and 8.1 nM in BMDMs and HMDMs, respectively.
in vitro	MCC950 specifically inhibits NLPR3 inflammasome activation.^[1]
in vivo	Inhibition of NLRP3 with MCC950 ameliorates proteinuria, renal histological lesions and podocyte foot process effacement in lupus-prone mice.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	NZM2328 podocyte cell line
	濃度	1 µM
	反応時間	24 h
	実験の流れ	The NZM2328 podocyte cell line was stimulated with a control serum or a serum from diseased NZM2328 mice or serum from diseased NZM2328 mice depleted of IgG, with or without MCC950 or Mito TEMPO for 24 hours. After this incubation, cells were subjected to flow cytometry and Western blot analyses for NLRP3 inflammasome activation, mitochondrial membrane potential and production of reactive oxygen species (ROS) analysis. (Data sourced from selleck products)
動物実験	動物モデル	female NZM2328, a new NZM strain produced by extended backcrosses of NZB/WF1 to NZW mice
	投薬量	10mg/kg
	投与方法	i.p. (Data sourced from selleck products)

参考

https://pubmed.ncbi.nlm.nih.gov/28544564/
https://pubmed.ncbi.nlm.nih.gov/35294888/
https://pubmed.ncbi.nlm.nih.gov/36087465/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Exosomal transfer of pro-pyroptotic miR-216a-5p exacerbates anthracycline cardiotoxicity through breast cancer-heart pathological crosstalk [ Signal Transduct Target Ther, 2025, 10(1):157]	PubMed: 40360476
Discovery of potent and selective inhibitors of human NLRP3 with a novel mechanism of action [ J Exp Med, 2025, 222(11)e20242403]	PubMed: 40892070
PM2.5 exacerbates nasal epithelial barrier dysfunction in allergic rhinitis by inducing NLRP3-mediated pyroptosis via the AhR/CYP1A1/ROS axis [ J Hazard Mater, 2025, 492:138145]	PubMed: 40209413
Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity [ J Immunother Cancer, 2025, 13(10)e012182]	PubMed: 41052880
Monocyte membrane-coated drug nanocrystals for enhanced targeted therapy of rheumatoid arthritis [ J Control Release, 2025, 384:113919]	PubMed: 40460929
Resibufogenin protects against atherosclerosis in ApoE-/- mice through blocking NLRP3 inflammasome assembly [ J Adv Res, 2025, S2090-1232(25)00272-3]	PubMed: 40258472
LL37 complexed to double-stranded RNA induces RIG-I-like receptor signalling and Gasdermin E activation facilitating IL-36γ release from keratinocytes [ Cell Death Dis, 2025, 16(1):198]	PubMed: 40121229
A small molecule directly targets NLRP3 to promote inflammasome activation and antitumor immunity [ Cell Death Dis, 2025, 16(1):252]	PubMed: 40185713
NETs activate the GAS6-AXL-NLRP3 axis in macrophages to drive morphine tolerance [ Cell Commun Signal, 2025, 23(1):181]	PubMed: 40217343
The role and mechanism of the NLRP3-IL-1β/IL-18 signaling axis in the progression of sepsis under an aging phenotype [ Life Sci, 2025, S0024-3205(25)00447-3]	PubMed: 40532971

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。