MDL-28170

製品コードS7394 バッチS739401

印刷

化学情報

Chemical Structure Synonyms Calpain Inhibitor III Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C22H26N2O4
分子量 382.45 CAS No. 88191-84-8
Solubility (25°C)* 体外 DMSO 76 mg/mL (198.71 mM)
Ethanol 10 mg/mL (26.14 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO Corn oil
2.7mg/ml Taking the 1 mL working solution as an example, add 50 μL of 54 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 MDL-28170 (Calpain Inhibitor III) is a potent and selective calpain inhibitor of calpain that penetrates the blood-brain barrier and inhibits brain cysteine protease activity after systemic administration. MDL-28170 is also an inhibitor of γ-secretase.
in vitro

MDL28170 significantly enhances Schwann cells (SCs) survival in vitro in response to oxidative stress induced by application of H2O2, without reducing LDH release.[3]
in vivo

MDL 28170 is a calpain inhibitor that protects against cortical neuronal damage even if the treatment is delayed until 3 h after reperfusion. However, the neuroprotective effect of this agent is less pronounced in the hippocampal CA1 sector.[1]
密度 g/mL

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Schwann cells
濃度 50 nM, 500 nM, 5 μM, 50 μM
反応時間 4 h
実験の流れ

To test the ability of MDL28170 to inhibit H2O2-mediated necrosis. Cells are treated with MDL28170 (50 nM, 500 nM, 5 μM, or 50 μM). After 1 h of drug pretreatment, fresh solution of drugs and H2O2 is added to the cells for the duration of the experiment. After 3 h of exposure the number of live or dead cells is assayed.
動物実験 動物モデル Male Mongolian gerbils
投薬量 50 mg/kg
投与方法 IP

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Astragaloside IV mitigates hypoxia-induced cardiac hypertrophy through calpain-1-mediated mTOR activation [ Phytomedicine, 2024, 125:155250] PubMed: 38295664
Ginsenoside Rg1 attenuates diabetic vascular endothelial dysfunction by inhibiting the calpain-1/ROS/PKC-β axis [ Life Sci, 2023, 329:121972] PubMed: 37482213
Prevention of noise-induced hearing loss by calpain inhibitor MDL-28170 is associated with upregulation of PI3K/Akt survival signaling pathway [ Front Cell Neurosci, 2023, 17:1199656] PubMed: 37484825
Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway [ J Ginseng Res, 2023, 47(1):144-154] PubMed: 36644390
DNA damage contributes to age-associated differences in SARS-CoV-2 infection [ Aging Cell, 2022, 21(12):e13729] PubMed: 36254583
Protective effect of Astragaloside IV on chronic intermittent hypoxia-induced vascular endothelial dysfunction through the calpain-1/SIRT1/AMPK signaling pathway [ Front Pharmacol, 2022, 13:920977] PubMed: 35983375
Inhibiting SRC activity attenuates kainic-acid induced mouse epilepsy via reducing NR2B phosphorylation and full-length NR2B expression [ Epilepsy Res, 2022, 185:106975] PubMed: 35907325

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。