Medroxyprogesterone

製品コードS3635 バッチS363502

印刷

化学情報

Chemical Structure Synonyms MP Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C22H32O3
分子量 344.49 CAS No. 520-85-4
Solubility (25°C)* 体外 DMSO 69 mg/mL (200.29 mM)
Ethanol 14 mg/mL (40.63 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Medroxyprogesterone (MP) is a synthetic pregnane steroid and a derivative of progesterone. It is a potent progesterone receptor agonist.
in vitro Medroxyprogesterone acetate (MPA, 10 nM) treatment for 48 h induces proliferation of the cells (1.6-fold induction). MPA induces cyclin D1 expression (3.3-fold induction). MPA increases both the protein level (2.2-fold induction) and promoter activity (2.7-fold induction) of cyclin D1 in MCF-7 cells transfected with PRB but not with PRA. Although MPA transcriptionally activates cyclin D1 expression, cyclin D1 promoter does not have progesterone-responsive element-related sequence. MPA induces the transient phosphorylation of Akt (2.7-fold induction at 5 min) and also phosphorylation of inhibitor of NFkappaBalpha (IkappaBalpha) (2.3-fold induction)[1]. MPA activates glucocorticoid receptors, which could mimic part of the anti-atherosclerotic effects of glucocorticoids, and has, on the other hand, also anti-androgenic activity, which might diminish protective oestrogen effects[2].
in vivo Long-term treatment with MPA and MPA + E2 increases arterial thrombosis despite inhibitory effects of MPA on atherosclerosis in ApoE-deficient mice. Increased thrombin formation, reduced smooth muscle content and remodelling of non-collagenous plaque matrix may be involved in the pro-thrombotic effects. Thus, MPA exhibits differential effects on arterial thrombosis and on atherosclerosis. In monkeys, MPA interferes with anti-atherosclerotic oestrogen effects whereas progesterone does not[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 T47D human breast cancer cells
濃度 10 nM
反応時間 4 days
実験の流れ The cells are plated at a density of 10 or 40 × 104 cells/well in 12-well plates and allowed to attach overnight. The cells are then growth arrested by incubation in phenol red-free DMEM with 10% charcoal-stripped serum for 24 h followed by treatment with vehicle or MPA by exchanging the culture medium containing these agents with fresh medium every 24 h for 4 d. A Neubauer chamber is used to count cells, and trypan blue experiments are carried out in quadruplicate.

参考

  • https://pubmed.ncbi.nlm.nih.gov/16123159/
  • https://pubmed.ncbi.nlm.nih.gov/20050187/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。