Melphalan

製品コードS8266 バッチS826602

印刷

化学情報

Chemical Structure Synonyms Alkeran, Sarcolysin, L-PAM Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C13H18Cl2N2O2
分子量 305.20 CAS No. 148-82-3
Solubility (25°C)* 体外 DMSO 3.5 mg/mL (11.46 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Melphalan (Alkeran, Sarcolysin, L-PAM) is a phenylalanine derivative of nitrogen mustard with antineoplastic activity.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
in vitro Exposure of a myeloma cell line (RPMI 8226) to a 30-minute pulse of melphalan (1-phenylalanine-mustard) results in a cell cycle progression delay characteristic for DNA cross-linking agents[1]. This compound has been shown to bind to DNA, RNA, and protein in cells in vitro. It induces chromosomal aberrations, sister chromatid exchange, micronuclei, mutations at the HPRT gene, and DNA damage in human cells in vitro. It also induces transformation of C3H 10T1/2 and other cells. In cultured rodent cells, it induces chromosomal aberrations, sister chromatid exchange, gene mutations, and DNA damage. In addition, it induces aneuploidy and sex-linked recessive lethal mutations in Drosophila, and mutation in bacteria[3].
in vivo Melphalan has been tested in mice by oral, intraperitoneal, and dermal application; in rats by intraperitoneal injection, and in monkeys by oral administration. In mice, the administration of this compound produced forestomach papillomas, lymphosarcomas, and skin and lung tumours. In rats, it caused mammary gland tumours, and peritoneal sarcomas. Results in monkeys were inconclusive[3].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 human myeloma cell line RPMI 8226
濃度
反応時間 30 min
実験の流れ Cells are cultured in RPMI 1640 medium supplemented with 10% fetal calf serum (FCS), 1 % L-glutamine, 50 μg/ml of penicillin and 50 μg/ml of streptomycin. Cell viability is assessed by trypan blue exclusion. For the melphalan exposure, cells are collected from exponentially growing cell cultures. The cells were treated by a 30-min pulse of this compound in serum-free medium after which they are run in complete culture medium as described above without drug for various times.
動物実験 動物モデル Tg.AC mice
投薬量 0.25, 1, and 4 mg/kg weekly
投与方法 Topical administration/oral gavage

参考

  • https://pubmed.ncbi.nlm.nih.gov/1915798/
  • https://pubmed.ncbi.nlm.nih.gov/11695563/
  • https://www.ncbi.nlm.nih.gov/books/NBK304320/

カスタマーフィードバック

Data from [Data independently produced by , , Blood, 2016, 127(9):1138-50. ]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Perturb-Multimodal: A platform for pooled genetic screens with imaging and sequencing in intact mammalian tissue [ Cell, 2025, S0092-8674(25)00572-0] PubMed: 40513557
The de novo DNA methyltransferase 3B is a novel epigenetic regulator of MYC in multiple myeloma, representing a promising therapeutic target to counter relapse [ J Exp Clin Cancer Res, 2025, 44(1):125] PubMed: 40241199
Targeting the MARCH5-MFN2 axis to enhance mitochondrial fusion and sensitize multiple myeloma cells to venetoclax [ J Transl Med, 2025, 23(1):917] PubMed: 40814067
A platform for multimodal in vivo pooled genetic screens reveals regulators of liver function [ bioRxiv, 2025, 2024.11.18.624217] PubMed: 39605605
Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib [ Cell Death Dis, 2024, 15(8):588] PubMed: 39138149
Pro-survival signaling regulates lipophagy essential for multiple myeloma resistance to stress-induced death [ Cell Rep, 2024, 43(7):114445] PubMed: 38968073
DNp73 enhances tumor progression and immune evasion in multiple myeloma by targeting the MYC and MYCN pathways [ Front Immunol, 2024, 15:1470328] PubMed: 39380995
Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors [ iScience, 2024, 27(10):110862] PubMed: 39319271
Therapeutic potential of anti-PIK3CG treatment for multiple myeloma via inhibiting c-Myc pathway [ Heliyon, 2024, 10(1):e23165] PubMed: 38163179
Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastoma [ Cell Rep Med, 2023, 4(6):101091] PubMed: 37343516

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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