Metformin

製品コードS5958 バッチS595801

印刷

化学情報

Chemical Structure Synonyms 1,1-Dimethylbiguanide Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C4H11N5
分子量 129.16 CAS No. 657-24-9
Solubility (25°C)* 体外 DMSO 26 mg/mL (201.3 mM)
Water 26 mg/mL (201.3 mM)
Ethanol 26 mg/mL (201.3 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Metformin (1,1-Dimethylbiguanide), a widely used drug for treatment of type 2 diabetes, activates AMP-activated protein kinase (AMPK) in hepatocytes. Metformin promotes mitophagy in mononuclear cells. Metformin induces apoptosis of lung cancer cells through activating JNK/p38 MAPK pathway and GADD153.
in vitro

Metformin upregulates BAX activation with facilitation of BIM, BAD and PUMA, downregulates Bcl-2 and Bcl-xl, also induces cytochrome c release from mitochondria into the cytoplasm, directly triggering caspase-9-mediated mitochondrial apoptosis.[4]
in vivo

Metformin shows therapeutic effect of metformin in mouse model of liver fibrosis associated with nonalcoholic steatohepatitis (NASH) in which hepatic function, NASH lesions and fibrosis are improved.[4]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 human hepatic stellate cells (HSCs) LX-2, rat HSCs T6, human hepatocyte L02 and rat hepatocyte BRL-3A
濃度 0-80 mM
反応時間 24 h
実験の流れ

The human hepatic stellate cells (HSCs) LX-2 is maintained in RPMI 1640 medium, rat HSCs T6, human hepatocyte L02 and rat hepatocyte BRL-3A were maintained in DMEM, which are both supplemented with 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin sulfate at 37 ℃ in a humidified 5% CO2 incubator. Metformin and AICAR solutions are filtered through 0.22 μm millipore membrane filter units. CCK8 assay is used to detect the effects of different concentration of metformin and AICAR on the proliferation of hepatic stellate cells. T6 cells and LX-2 cells are treated with different concentration of metformin and AICAR for 24 h, and the expression of collagen and α - SMA protein are detected by Western blot. Cells are treated with 10 mM metformin and 0.5 mM AICAR for 24 h, and the expression of collagen and α - SMA protein are detected by Western blot. The mRNA levels of collagen I and α - SMA are detected by RT-qPCR.
動物実験 動物モデル Male C57BL/6N mice
投薬量 10-200 mg/kg; 65 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Pump-Less, Recirculating Organ-On-Chip (rOoC) Platform To Model The Metabolic Crosstalk Between Islets and Liver [ Adv Healthc Mater, 2024, e2303785.] PubMed: 38221504
A novel direct adenosine monophosphate kinase activator ameliorates disease progression in preclinical models of Autosomal Dominant Polycystic Kidney Disease [ Kidney Int, 2023, 103(5):917-929] PubMed: 36804411
Metformin suppresses cardiac fibroblast proliferation under high-glucose conditions via regulating the mitochondrial complex I protein Grim-19 involved in the Sirt1/Stat3 signaling pathway [ Free Radic Biol Med, 2023, 206:1-12] PubMed: 37353174
AMPK activator decelerates osteoarthritis development by inhibition of β-catenin signaling in chondrocytes [ J Orthop Translat, 2023, 38:158-166] PubMed: 36439631
Resistin secreted by porcine alveolar macrophages leads to endothelial cell dysfunction during Haemophilus parasuis infection [ Virulence, 2023, 14(1):2171636] PubMed: 36694280
Resveratrol Upregulates Senescence Marker Protein 30 by Activating AMPK/Sirt1-Foxo1 Signals and Attenuating H2O2-Induced Damage in FAO Rat Liver Cells [ J Nutr Sci Vitaminol (Tokyo), 2023, 10.3177/jnsv.69.388] PubMed: 37940580
The NCF1 variant p.R90H aggravates autoimmunity by facilitating the activation of plasmacytoid dendritic cells [ The Journal of Clinical Investigation, 2022-, 132(16)] PubMed: None
Integrative analysis of drug response and clinical outcome in acute myeloid leukemia [ Cancer Cell, 2022, S1535-6108(22)00312-9] PubMed: 35868306
Disrupting metformin adaptation of liver cancer cells by targeting the TOMM34/ATP5B axis [ EMBO Mol Med, 2022, 14(12):e16082] PubMed: 36321555
Hepatic Leptin Signaling Improves Hyperglycemia by Stimulating MAPK Phosphatase-3 Protein Degradation via STAT3 [ Cell Mol Gastroenterol Hepatol, 2022, S2352-345X(22)00168-0] PubMed: 35863745

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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