Metformin

製品コードS5958 バッチS595805

印刷

化学情報

Synonyms

1,1-Dimethylbiguanide

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₄H₁₁N₅

分子量

129.16

CAS No.

657-24-9

Solubility (25°C)*

体外

Water

130 mg/mL (1006.5 mM)

DMSO

26 mg/mL (201.3 mM)

Ethanol

26 mg/mL (201.3 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.300mg/ml (10.07mM)	Taking the 1 mL working solution as an example, add 50 μL of clarified DMSO stock solution of 26 mg/ml to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Metformin (1,1-Dimethylbiguanide), a widely used drug for treatment of type 2 diabetes, activates AMP-activated protein kinase (AMPK) in hepatocytes. Metformin promotes mitophagy in mononuclear cells. Metformin induces apoptosis of lung cancer cells through activating JNK/p38 MAPK pathway and GADD153.
in vitro	Metformin upregulates BAX activation with facilitation of BIM, BAD and PUMA, downregulates Bcl-2 and Bcl-xl, also induces cytochrome c release from mitochondria into the cytoplasm, directly triggering caspase-9-mediated mitochondrial apoptosis.^[4]
in vivo	Metformin shows therapeutic effect of this compound in mouse model of liver fibrosis associated with nonalcoholic steatohepatitis (NASH) in which hepatic function, NASH lesions and fibrosis are improved.^[4]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	human hepatic stellate cells (HSCs) LX-2, rat HSCs T6, human hepatocyte L02 and rat hepatocyte BRL-3A
	濃度	0-80 mM
	反応時間	24 h
	実験の流れ	The human hepatic stellate cells (HSCs) LX-2 is maintained in RPMI 1640 medium, rat HSCs T6, human hepatocyte L02 and rat hepatocyte BRL-3A were maintained in DMEM, which are both supplemented with 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin sulfate at 37 ℃ in a humidified 5% CO₂incubator. Metformin and AICAR solutions are filtered through 0.22 μm millipore membrane filter units. CCK8 assay is used to detect the effects of different concentration of this compound and AICAR on the proliferation of hepatic stellate cells. T6 cells and LX-2 cells are treated with different concentration of this chemical and AICAR for 24 h, and the expression of collagen and α - SMA protein are detected by Western blot. Cells are treated with 10 mM this compound and 0.5 mM AICAR for 24 h, and the expression of collagen and α - SMA protein are detected by Western blot. The mRNA levels of collagen I and α - SMA are detected by RT-qPCR.
動物実験	動物モデル	Male C57BL/6N mice
	投薬量	10-200 mg/kg; 65 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/11602624/
https://pubmed.ncbi.nlm.nih.gov/31225649/
https://pubmed.ncbi.nlm.nih.gov/21895401/

https://pubmed.ncbi.nlm.nih.gov/35344814/

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

High fructose consumption aggravates inflammation by promoting effector T cell generation via inducing metabolic reprogramming [ Signal Transduct Target Ther, 2025, 10(1):271]	PubMed: 40854873
The first abdominal aortic aneurysm organoid model replicates complex microenvironment for in vitro disease study [ Theranostics, 2025, 15(17):9029-9046]	PubMed: 40963926
Enhancing CAR-T Cell Metabolic Fitness and Memory Phenotype for Improved Efficacy against Hepatocellular Carcinoma [ Int J Biol Sci, 2025, 21(9):4231-4251]	PubMed: 40612678
TNF-α modulates cell proliferation via SOX4/TGF-β/Smad signaling in benign prostatic hyperplasia [ Cell Death Dis, 2025, 16(1):472]	PubMed: 40595502
Addition of metformin to anti-PD-1/PD-L1 drugs activates anti-tumor immune response in peripheral immune cells of NSCLC patients [ Cell Death Dis, 2025, 16(1):286]	PubMed: 40221409
Stromal Cell Derived Factor-1 Promotes Hepatic Insulin Resistance via Inhibiting Hepatocyte Lipophagy [ J Cell Mol Med, 2025, 29(2):e70352]	PubMed: 39855896
PGC-1α activation to enhance macrophage immune function in mycobacterial infections [ PLoS One, 2025, 20(2):e0310908]	PubMed: 39913377
Metformin alleviates lung ischemia‑reperfusion injury via the SIRT1 pathway following lung transplantation in diabetic rats [ Mol Med Rep, 2025, 32(5)287]	PubMed: 40808338
Nuclear PD-L1 compartmentalization suppresses tumorigenesis and overcomes immunocheckpoint therapy resistance in mice via histone macroH2A1 [ J Clin Invest, 2024, 134(22)e181314]	PubMed: 39545415
Pump-Less, Recirculating Organ-On-Chip (rOoC) Platform To Model The Metabolic Crosstalk Between Islets and Liver [ Adv Healthc Mater, 2024, e2303785.]	PubMed: 38221504

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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