受注:045-509-1970 |
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Synonyms | Amethopterin disodium; CL14377 disodium; WR19039 disodium | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C20H20N8O5.2Na |
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分子量 | 498.4 | CAS No. | 7413-34-5 | |
Solubility (25°C)* | 体外 | Water | 99 mg/mL (198.63 mM) | |
DMSO | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Methotrexate disodium, an analog of folic acid, is a nonspecific inhibitor of the dihydrofolate reductase(DHFR) of bacteria and cancerous cells as well as normal cells. It forms an inactive ternary complex with DHFR and NADPH. Methotrexate (MTX) induces apoptosis. |
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in vitro | Methotrexate (0.1-10 mM) induces apoptosis of in vitro activated T cells from human peripheral blood. Methotrexate achieves clonal deletion of activated T cells in mixed lymphocyte reactions. Methotrexate can selectively delete activated peripheral blood T cells by a CD95-independent pathway. [1] Methotrexate is taken up by cells via the reduced folate carrier and then is converted within the cells to polyglutamates. Methotrexate leads to diminished production of leukotriene B4 by neutrophils stimulated ex vivo. Methotrexate polyglutamates inhibit the enzyme aminoimidazolecarboxamidoadenosineribonucleotide (AICAR) transformylase more potently than the other enzymes involved in purine biosynthesis. Methotrexate is also known to suppress TNF activity by suppressing TNF-induced nuclear factor-κB activation in vitro, in part related to a reduction in the degradation and inactivation of an inhibitor of this factor, IκBα, and probably related to the release of adenosine. Methotrexate suppresses the production of both TNF and IFN-γ by T-cell-receptor-primed T lymphocytes from both healthy human donors and RA patients. Methotrexate treatment is associated with a significant decrease of TNF-α-positive CD4+ T cells, while the number of T cells expressing the anti-inflammatory cytokine IL-10 increased. [2] |
in vivo | Methotrexate increases splenocyte AICAR content, raised adenosine concentrations in exudates from carrageenan-inflamed air pouches, and markedly inhibits leukocyte accumulation in inflamed air pouches in mice. Methotrexate-mediated reduction in leukocyte accumulation is partially reversed by injection of adenosine deaminase (ADA) into the air pouch, completely reverses by a specific adenosine A2 receptor antagonist, 3,7-dimethyl-1-propargylxanthine (DMPX), but not affected by an adenosine A1 receptor antagonist, 8-cyclopentyl-dipropylxanthine in mice. [3] |
Oncogenic β-catenin-driven liver cancer is susceptible to methotrexate-mediated disruption of nucleotide synthesis [ Chin Med J (Engl), 2023, 10.1097/CM9.0000000000002816] | PubMed: 37612257 |
Modulation of Skin Inflammatory Responses by Aluminum Adjuvant [ Pharmaceutics, 2023, 15(2)576] | PubMed: 36839900 |
Multi-omics analysis based on 3D-bioprinted models innovates therapeutic target discovery of osteosarcoma [ Bioact Mater, 2022, 18:459-470] | PubMed: 35415297 |
Metabolic control of CD47 expression through LAT2-mediated amino acid uptake promotes tumor immune evasion [ Nat Commun, 2022, 13(1):6308] | PubMed: 36274066 |
Hydroxychloroquine lowers Alzheimer's disease and related dementias risk and rescues molecular phenotypes related to Alzheimer's disease [ Mol Psychiatry, 2022, none] | PubMed: 36577843 |
Addressing Doxorubicin Resistance in Bone Sarcomas Using Novel Drug-Resistant Models [ Int J Mol Sci, 2022, 23(12)6425] | PubMed: 35742867 |
Lectin galactoside-binding soluble 3 binding protein mediates methotrexate resistance in choriocarcinoma cell lines [ Bioengineered, 2022, 13(2):2076-2086] | PubMed: 35038949 |
A network-based approach to integrate nutrient microenvironment in the prediction of synthetic lethality in cancer metabolism [ PLoS Comput Biol, 2022, 18(3):e1009395] | PubMed: 35286311 |
A study of protein-drug interaction based on solvent-induced protein aggregation by fluorescence correlation spectroscopy [ Analyst, 2022, 10.1039/d2an00031h] | PubMed: 35253833 |
The novel mechanism of Med12-mediated drug resistance in a TGFBR2-independent manner [ Biochem Biophys Res Commun, 2022, 610:1-7] | PubMed: 35461070 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。