Methyl-β-cyclodextrin (MβCD)

製品コードS6827 バッチS682701

印刷

化学情報

Chemical Structure Synonyms Methyl-beta-cyclodextrin, M-β-CyD, Methyl-β-CD Storage
(From the date of receipt)		 3 years -20°C powder
化学式
分子量 CAS No. 128446-36-6
Solubility (25°C)* 体外 Water 100 mg/mL
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

生物活性

製品説明 メチル-β-シクロデキストリン（MβCD）は、主に脂質ラフト破壊に使用されるコレステロール除去剤です。メチル-β-シクロデキストリンは、PEL細胞においてカスパーゼ依存性アポトーシスを誘導し、明らかな副作用なしにPEL細胞の増殖と浸潤を阻害します。これは、潜在的な有効な抗腫瘍活性を示唆しています。
in vitro

Methyl-β-cyclodextrin (MβCD) inhibits SDF-1α-induced platelet aggregation and Akt phosphorylation by raft disruption.
in vivo

In a Primary effusion lymphoma (PEL) xenograft mouse model, Methyl-β-cyclodextrin (MβCD) significantly inhibits the growth and invasion of PEL cells without apparent adverse effects. Mice treated with this compound appear to be healthy, whereas non-treated mice have a distended abdominal region. The body weights of control are significantly higher than those of the treated mice. It also results in a significantly lower volume of ascites than that of non-treated mice.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Platelets
濃度 2%
反応時間 15 min
実験の流れ

Freshly isolated human platelets are incubated with Methyl-β-cyclodextrin (MβCD) and SDF-1α, then centrifuged for 1 min at 3000 rpm. The resulting pellet is resuspended and boiled in Laemmli sample buffer containing phosphatase inhibitor cocktails 2 and 3. Phosphorylations of Akt (Ser473) is detected by western blotting. Platelet aggregation is measured as the optical density of PRP during aggregation using a platelet aggregometer, with the platelet-poor plasma 100% standard for change in optical density.
動物実験 動物モデル PEL xenograft mouse model (NOD/Rag-2/Jak3-double deficient (NRJ) mice bearing BCBL-1 cells
投薬量 500 mg/kg
投与方法 i.p

参考

  • https://pubmed.ncbi.nlm.nih.gov/25446086/
  • https://pubmed.ncbi.nlm.nih.gov/28072855/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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