Methyl-β-cyclodextrin (MβCD)

製品コードS6827 バッチS682701

印刷

化学情報

Synonyms

Methyl-beta-cyclodextrin, M-β-CyD, Methyl-β-CD

Storage
(From the date of receipt)

3 years -20°C powder

化学式

分子量

CAS No.

128446-36-6

Solubility (25°C)*

体外

Water

100 mg/mL

体内（毎回新しく調製した物を用意してください）

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	5.000mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.500mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

生物活性

製品説明	Methyl-β-cyclodextrin (MβCD) is a cholesterol-removing agent mainly used for lipid raft disruption. Methyl-β-cyclodextrin induces caspase-dependent apoptosis in PEL cells and inhibits the growth and invasion of PEL cells without apparent adverse effects, suggesting the potential effective antitumor activities.
in vitro	By raft disruption, Methyl-β-cyclodextrin (MβCD) inhibits SDF-1α-induced platelet aggregation and Akt phosphorylation.^[2]
in vivo	In a Primary effusion lymphoma (PEL) xenograft mouse model, Methyl-β-cyclodextrin (MβCD) significantly inhibits the growth and invasion of PEL cells without apparent adverse effects. Treated mice appear healthy, whereas non-treated counterparts exhibit a distended abdominal region. The body weights of control animals are significantly higher than those receiving this compound. Additionally, it results in a significantly lower volume of ascites compared to non-treated mice.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Platelets
	濃度	2%
	反応時間	15 min
	実験の流れ	Freshly isolated human platelets are incubated with Methyl-β-cyclodextrin (MβCD) and SDF-1α, then centrifuged for 1 min at 3000 rpm. The resulting pellet is resuspended and boiled in Laemmli sample buffer containing phosphatase inhibitor cocktails 2 and 3. Phosphorylations of Akt (Ser473) is detected by western blotting. Platelet aggregation is measured as the optical density of PRP during aggregation using a platelet aggregometer, with the platelet-poor plasma 100% standard for change in optical density.
動物実験	動物モデル	PEL xenograft mouse model (NOD/Rag-2/Jak3-double deficient (NRJ) mice bearing BCBL-1 cells
	投薬量	500 mg/kg
	投与方法	i.p

参考

https://pubmed.ncbi.nlm.nih.gov/25446086/
https://pubmed.ncbi.nlm.nih.gov/28072855/

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Real-Time Dissection of the Transportation and miRNA-Release Dynamics of Small Extracellular Vesicles [ Adv Sci (Weinh), 2023, 10(7):e2205566]	PubMed: 36599707
Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling [ Cancer Sci, 2023, 114(11):4252-4269]	PubMed: 37671589
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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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