Methyl Dihydrojasmonate

化学情報

生物活性

プロトコル(参考用のみ)

参考

製品コードS5139 バッチS513901

	Synonyms	Hedione, Kharismal	Storage (From the date of receipt)	2 years -80°C liquid
	化学式	C₁₃H₂₂O₃
	分子量	226.31	CAS No.	24851-98-7
Solubility (25°C)*	体外


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

細胞アッセイ	細胞株	MCF-7 cells
	濃度	0, 5, 12.5, 25, 50 μl/ml
	反応時間	48 h
	実験の流れ	Measurement of potential cytotoxicity is performed by SRB assay where Cells are plated in 96-multiwell plate (10⁴ cells/well) for 24 h before exposure to the compound(s) to allow attachment of the cells to the wall of the plate. Different concentrations of the compound under test; 0, 5, 12.5, 25, 50 μl/ml for the Pure MDHJ (Oily liquid) and 0, 25, 50, 75, 100 μl/ml for the used formulation are added to the cell monolayer triplicate wells; which are prepared for each individual concentration. A monolayer cells are incubated together with the compound(s) for 48 h at 37 °C and in atmosphere of 5% CO₂. After 48 h, cells are fixed, washed and stained with Sulfo-Rhodamine-B stain (SRB). Excess stain is washed with acetic acid and the attached stain is recovered with Tris EDTA buffer. Color intensity is measured utilizing an ELISA reader.
動物実験	動物モデル	Sprague-Dawley rats
	投薬量	0, 40, 80 or 120 mg/kg/day
	投与方法	oral

Methyl dihydrojasmonate (Hedione, Kharismal) is a flavouring ingredien with the smell vaguely similar to jasmin.

in vitro

Methyl dihydrojasmonate inhibits the growth of leukemia cells as well as lung, breast, and prostate cancer cells. It opens the mitocondrial permeability transition-pore complex, allowing cytochrome C release and induction of apoptosis, and the expression of reactive oxygen species. Jasmonate-derivative compounds, including jasmonic acid (JA) and methyl dihydrojasmonate (MJ), also induces the suppression of cell proliferation in human and mouse cancer cell lines, such as prostate cancer and breast cancers, melanoma, leukemia, lymphoid, and myeloid^[2].

Methyl dihydrojasmonate (MDHJ) is a promising anti-cancer drug of a plant origin (jasmine plant)^[1]. Jasmonate-derivative compounds are poorly water-soluble, and cannot be administered intravenously or adequately absorbed by the body^[2]. The oral LD50 of Methyl dihydrojasmonate in rats exceeds 5.0 g/kg. By the dermal route, the rabbit LD50 also exceeds 5.0 g/kg. By the intraperitoneal route in ICR mice, the LD50 is 1397.2 mg/kg. While in rats, an intraperitoneal injection of Methyl dihydrojasmonate in corn oil produces clinical signs at 1000 mg/kg and mortality at 2000 mg/kg. Methyl dihydrojasmonate is not considered to possess a mutagenic or genotoxic potential^[3].

0.998 g/mL at 25 °C

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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