Methyl gallate

製品コードS3790 バッチS379001

化学情報

	Synonyms	Methylgallate, Gallic acid methyl ester, Gallicin	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₈H₈O₅
	分子量	184.15	CAS No.	99-24-1
Solubility (25°C)*	体外	DMSO	36 mg/mL (195.49 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Methyl Gallate (Methylgallate, Gallic acid methyl ester, Gallicin) is a plant polyphenol with antioxidant, anticancer, and anti-inflammatory activities.
in vitro	Methyl gallate inhibits Treg cell-suppressive effects on effector CD4+ T cells and Treg migration toward tumor environment. The expression of Treg surface markers including CTLA-4, CCR4, CXCR4, and glucocorticoid-induced TNFR are significantly suppressed upon methyl gallate treatment^[2].
in vivo	In tumor-bearing hosts, methyl gallate treatment substantially reduces tumor growth and prolonged the survival rate. In contrast, nu/nu mice do not show decreased tumor progression in response to methyl gallate. In addition, in tumor-bearing Treg-depleted mice, tumor growth and the survival rates are not changed by methyl gallate treatment, strongly suggesting that the main therapeutic target of methyl gallate in tumor suppression is related to modulation of the CD4+CD25+ Treg cell functions. In the spleen of tumor-bearing mice, methyl gallate treatment induces a significant decrease in the CD4+CD25+Foxp^3high Treg cell population. Especially, the number of tumor-infiltrating CD25+Foxp^3high Treg cells is significantly lower in methyl gallate-treated mice. methyl gallate can be used to reverse immune suppression and as a potentially useful adjunct for enhancing the efficacy of immune-based cancer therapy^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HUVEC
	濃度	0-0.5 mM
	反応時間	1 h
	実験の流れ	HUVECs are grown in 96-well micro plates in a final volume of 100 μl culture medium containing 10⁴ cells per well. After allowing the cultures to grow to confluence, chemical and H2O2 treatments are followed with certain intervals. To determine the cell viability, XTT assay kit is used. Two cases of oxidative stress are applied to pre-incubated HUVECs with chemical for 1 h: the treatment with 1 mM H2O2 for 1 h and the treatment with 0.1 mM H2O2 for 48 h. Concentrations of chemical are varied from 0 to 0.5 mM.
動物実験	動物モデル	C57BL/6, Foxp3^EGFPC57BL/6 or nu/nu mice
	投薬量	200 μg
	投与方法	i.p.

参考

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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