MHY1485

製品コードS7811 バッチS781102

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C17H21N7O4
分子量 387.39 CAS No. 326914-06-1
Solubility (25°C)* 体外 DMSO 3.75 mg/mL (9.68 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.
in vitro

MHY1485 suppresses the basal autophagic flux. This compound causes the accumulation of LC3II and enlargement of the autophagosomes in a dose- and time- dependent manner.[1]

It increases phospho-mTOR levels and phosphorylation of downstream S6K1 and rpS6 proteins without affecting total mTOR content, total S6K1 and rpS6 levels. Short-term treatment of ovaries with this chemical followed by allo-transplantation promoted secondary follicle growth. Treatment with it and subsequent grafting allowed the derivation of mature oocytes and healthy offspring.[2]
in vivo

MHY1485 is a potent, and cell-permeable mTORC1 agonist.

プロトコル(参考用のみ)

キナーゼアッセイ Activation of mTOR
Western blot analysis is performed to detect the change of total protein level and levels of phosphorylated forms of mTOR and 4E-BP1 reflecting the activity of mTOR. Ac2F cells are treated with MHY1485 of different concentrations and rapamycin 5 mM as a positive control for 1 h. Cells are washed with cold PBS and harvested. Cell lysates are prepared using RIPA buffer. Protein concentration is determined by the bicinchoninic acid (BCA) method. Equal amounts of protein are separated on 10-12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. The gels are subsequently transferred onto a polyvinylidene difluoride membrane by electroblotting for 2 h at 60-75 V. The membranes are blocked in a 5% nonfat milk solution in Tris-buffered saline (TBS) with 0.5% Tween-20, and incubated with primary antibodies. Pre-stained protein markers are used for molecular-weight determination.
細胞アッセイ 細胞株 TU177 cells
濃度 10 µM
反応時間 24 h
実験の流れ

Cells were treated with indicated concentration of this compound for 24 h.
動物実験 動物モデル C57BL/6 mice
投薬量 5 mg/kg
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/22927967/
  • https://pubmed.ncbi.nlm.nih.gov/25710488/
  • https://pubmed.ncbi.nlm.nih.gov/36438491/
  • https://pubmed.ncbi.nlm.nih.gov/34580888/

カスタマーフィードバック

Data from [Data independently produced by , , FASEB J, 2018, 32(1):512-528]

Data from [Data independently produced by , , Int J Biol Sci, 2017, 13(11):1398-1408]

Data from [Data independently produced by , , Biochem J, 2017, 474(16):2733-2747]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Metformin attenuates alveolar bone destruction in mice with apical periodontitis and inhibits pro-inflammatory cytokine synthesis in lipopolysaccharide-stimulated RAW264.7 through the AMPK-mTOR-NF-κB pathway [ Front Immunol, 2025, 16:1643676] PubMed: 40821823
Natural polyphenol mangiferin delays neuronal cell senescence by inhibiting neuroinflammation mediated by microglial activation [ IBRO Neurosci Rep, 2025, 18:574-591] PubMed: 40271493
Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis [ Bone Res, 2024, 12(1):15] PubMed: 38433252
Augmented ERO1α upon mTORC1 activation induces ferroptosis resistance and tumor progression via upregulation of SLC7A11 [ J Exp Clin Cancer Res, 2024, 43(1):112] PubMed: 38610018
Gestational exposure to PM2.5 disrupts fetal development by suppressing placental trophoblast syncytialization via progranulin/mTOR signaling [ Sci Total Environ, 2024, 921:171101] PubMed: 38387595
Multi-omics analyses of cancer-linked clinical salmonellae reveal bacterial-induced host metabolic shift and mTOR-dependent cell transformation [ Cell Rep, 2024, 43(11):114931] PubMed: 39488829
Toosendanin alleviates acute lung injury by reducing pulmonary vascular barrier dysfunction mediated by endoplasmic reticulum stress through mTOR [ Phytomedicine, 2024, 136:156277] PubMed: 39615214
Tetrahydrocurcumin ameliorates hepatic steatosis by restoring hepatocytes lipophagy through mTORC1-TFEB pathway in nonalcoholic steatohepatitis [ Biomed Pharmacother, 2024, 178:117297] PubMed: 39137653
Adenosine kinase protects against acetaminophen-induced acute liver injury by activating autophagy in hepatocytes [ Cell Biol Toxicol, 2024, 40(1):59] PubMed: 39060559
Polyphyllin I alleviates neuroinflammation after cerebral ischemia-reperfusion injury via facilitating autophagy-mediated M2 microglial polarization [ Mol Med, 2024, 30(1):59] PubMed: 38745316

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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