Minocycline HCl

製品コードS4226 バッチS422604

化学情報

	Synonyms	CL 59806	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₃H₂₇N₃O₇.HCl
	分子量	493.94	CAS No.	13614-98-7
Solubility (25°C)*	体外	Water	6 mg/mL warmed with 50ºC water bath (12.14 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Minocycline HCl is the most lipid soluble and most active tetracycline antibiotic, binds to the 30S ribosomal subunit, preventing the binding of tRNA to the mRNA-ribosome complex and interfering with protein synthesis.
in vitro	Minocycline is a second-generation tetracycline used in humans, which effectively crosses the blood−brain barrier. It inhibits the activity of caspase-1, caspase-3, inducible form of nitric oxide synthetase (iNOS) and p38 mitogen-activated protein kinase (MAPK). After experimental ischemia, minocycline inhibits caspase-1 and inducible nitric oxide synthetase (iNOS) upregulation, and decreases infarct size. Minocycline inhibits mitochondrial permeability-transition-mediated cytochrome c release. Minocycline-mediated inhibition of cytochrome c release is demonstrated in vivo, in cells, and in isolated mitochondria. ^[2]
in vivo	Minocycline is a semi-synthetic tetracycline derivative which is well absorbed and distributed in body tissues and is suitable for twice daily administration. Minocycline's effects are related to the inhibition of protein synthesis. Although minocycline's broader spectrum of activity, compared to other members of the group, includes activity against Neisseria meningitidis, its use as a prophylaxis is no longer recomended because of side effects (dizziness and vertigo). ^[1] Minocycline is remarkable neuroprotective qualities in models of cerebral ischaemia, traumatic brain injury, and Huntington's and Parkinson's disease. The neuroprotective action of minocycline may include its inhibitory effect on 5-lipoxygenase, an inflammatory enzyme associated with brain aging. ^[3]

製品説明

Minocycline HCl is the most lipid soluble and most active tetracycline antibiotic, binds to the 30S ribosomal subunit, preventing the binding of tRNA to the mRNA-ribosome complex and interfering with protein synthesis.

in vitro

Minocycline is a second-generation tetracycline used in humans, which effectively crosses the blood−brain barrier. It inhibits the activity of caspase-1, caspase-3, inducible form of nitric oxide synthetase (iNOS) and p38 mitogen-activated protein kinase (MAPK). After experimental ischemia, minocycline inhibits caspase-1 and inducible nitric oxide synthetase (iNOS) upregulation, and decreases infarct size. Minocycline inhibits mitochondrial permeability-transition-mediated cytochrome c release. Minocycline-mediated inhibition of cytochrome c release is demonstrated in vivo, in cells, and in isolated mitochondria. ^[2]

in vivo

Minocycline is a semi-synthetic tetracycline derivative which is well absorbed and distributed in body tissues and is suitable for twice daily administration. Minocycline's effects are related to the inhibition of protein synthesis. Although minocycline's broader spectrum of activity, compared to other members of the group, includes activity against Neisseria meningitidis, its use as a prophylaxis is no longer recomended because of side effects (dizziness and vertigo). ^[1]

Minocycline is remarkable neuroprotective qualities in models of cerebral ischaemia, traumatic brain injury, and Huntington's and Parkinson's disease. The neuroprotective action of minocycline may include its inhibitory effect on 5-lipoxygenase, an inflammatory enzyme associated with brain aging. ^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	OVCAR-3, SKOV-3 and A2780 cells
	濃度	IC50 values of 62.0, 56.1 and 59.5 μM, respectively
	反応時間	72 h
	実験の流れ	Cells were treated with various concentrations of drug for 72 h.
動物実験	動物モデル	Female nude mice
	投薬量	10 or 30 mg/kg
	投与方法

参考

[4] Pourgholami MH, et al. Gynecol Oncol. 2012 May;125(2):433-40.

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

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Intratarget Microdosing for Deep Phenotyping of Multiple Drug Effects in the Live Brain [ Front Bioeng Biotechnol, 2022, 10:855755]	PubMed: 35372313
Microglial Activation Damages Dopaminergic Neurons through MMP-2/-9-Mediated Increase of Blood-Brain Barrier Permeability in a Parkinson's Disease Mouse Model [ Int J Mol Sci, 2022, 23(5)2793]	PubMed: 35269933
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Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs [ Front Cell Dev Biol, 2022, 10:840492]	PubMed: 35211474
Protective Effect of Minocycline Hydrochloride on the Mouse Embryonic Development Against Suboptimal Environment [ Front Cell Dev Biol, 2022, 10:799042]	PubMed: 35178387
Monophosphoryl lipid A tolerance against chronic stress-induced depression-like behaviors in mice [ Int J Neuropsychopharmacol, 2022, pyab097]	PubMed: 35015863

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人間や獣医の診断であるか治療的な使用のためにでない。

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