Mirin

製品コードS8096 バッチS809601

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₀H₈N₂O₂S

分子量

220.25

CAS No.

1198097-97-0

Solubility (25°C)*

体外

DMSO

44 mg/mL (199.77 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Mirin is a potent Mre11–Rad50–Nbs1 (MRN) complex inhibitor, and inhibits Mre11-associated exonuclease activity. Mirin inhibits MRN-dependent activation of ATM.
in vitro	Mirin inhibits DSB-induced ATM activation, the ATM-dependent phosphorylation of the downstream targets Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DSBs. This compound also inhibits the G2 checkpoint in TOSA4 cells, and homology-dependent DNA repair in HEK293 cells. ^[1] In cells with integrated HPV16 (SiHa), it sensitizes HPV episomes to PA25 resulting in a ∼5-fold reduction of the PA25 IC50. ^[2] Pretreatment with this chemical also decreases cell viability and inhibits proliferating cell nuclear antigen expression in cisplatin-treated human embryonic kidney 293 cells. ^[3]
in vivo	Mirin in nanoparticles resulted in a sharp impairment of tumor growth, associated with DDR activation, p53 accumulation, and cell death.

製品説明

Mirin is a potent Mre11–Rad50–Nbs1 (MRN) complex inhibitor, and inhibits Mre11-associated exonuclease activity. Mirin inhibits MRN-dependent activation of ATM.

in vitro

Mirin inhibits DSB-induced ATM activation, the ATM-dependent phosphorylation of the downstream targets Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DSBs. This compound also inhibits the G2 checkpoint in TOSA4 cells, and homology-dependent DNA repair in HEK293 cells. ^[1]

In cells with integrated HPV16 (SiHa), it sensitizes HPV episomes to PA25 resulting in a ∼5-fold reduction of the PA25 IC50. ^[2]

Pretreatment with this chemical also decreases cell viability and inhibits proliferating cell nuclear antigen expression in cisplatin-treated human embryonic kidney 293 cells. ^[3]

in vivo

Mirin in nanoparticles resulted in a sharp impairment of tumor growth, associated with DDR activation, p53 accumulation, and cell death.

プロトコル(参考用のみ)

キナーゼアッセイ	Nuclease assay
キナーゼアッセイ	Reactions with oligonucleotide nonhairpin substrates contains 25 mM MOPS (pH 7.0), 60 mM KCl, 0.2% Tween 20, 2 mM DTT, 1 mM or 5 MnCl₂ (or 5 mM MgCl₂, or 5 mM CaCl₂), 0.1 pmol of DNA substrate, and 0.3 pmol of Mre11 (or an equivalent amount of Mre11 complexed with Rad50) in a volume of 10 μl, and are incubated at 37°C for 30 min. SDS, EDTA, and proteinase K are then added to final concentrations of 0.2%, 5 mM, and 0.1 mg/ml, respectively, and incubated for another 15 min. 4 μl of each reaction is mixed with 4 μl of formamide loading buffer, and then loaded onto a sequencing gel containing 10% acrylamide and 7 M urea. After the run, each gel is analyzed using a phosphorimaging system. Reactions containing hairpin substrates are identical to those with nonhairpin substrates except that 3 pmol of this compound is added to reactions as indicated, and the reactions are incubated at room temperature overnight. Nonhomologous end-joining reactions contains 25 mM MOPS (pH 7.0), 60 mM KCl, 0.2% Tween 20, 2 mM DTT, 4 mM MgCl₂, 2 mM MnCl₂, 0.5 mM ATP, 4 ng of plasmid DNA, 10% polyethylene glycol, 0.01 pmol of human DNA ligase I, and 0.06 pmol of this chemical or 0.1 units of E. coli exonuclease III (GIBCO-BRL), in a volume of 10 μl. After incubation at 37°C for 25 min, Tween 20 is added to a final concentration of 0.5%, and a 2.5 μl aliquot is amplified by PCR using primers DAR5 and DAR147. PCR products are cloned using the TA cloning kit and sequenced using an automated ABI Capillary Genetic Analyzer.
細胞アッセイ	細胞株	HEK 293 cells
	濃度	100 μM
	反応時間	24 h
	実験の流れ	Human embryonic kidney (HEK) 293 cells are maintained in RPMI-1640 supplemented with 5% heat-inactivated fetal bovine serum, penicillin (100 U/mL), and streptomycin (100 mg/mL) in humidified air with 5% CO₂ at 37 °C. Cells are given fresh medium at 48 h intervals. The cells are seeded in 96-well plates in regular growth medium. Cells are pretreated with mirin (100 μM for 1 h before the cisplatin (20 μM) treatment followed by incubation for 8 and 24 h. The MTT assay is performed using the EZ-Cytox cell viability assay kit according to the manufacturer's protocol and MTT reduction is measured at a 450 nm wavelength using a micro-plate reader.
動物実験	動物モデル	Female BALB/c nude mice
	投薬量	50 mg/kg
	投与方法

参考

https://pubmed.ncbi.nlm.nih.gov/18176557/
https://pubmed.ncbi.nlm.nih.gov/24098381/
https://pubmed.ncbi.nlm.nih.gov/26056972/

https://pubmed.ncbi.nlm.nih.gov/30166519/

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カスタマーフィードバック

: Data from [Data independently produced by , , Aging, 2018, 10(4):549-560]

: Data from [Data independently produced by , , DNA Repair, 2018, 70:67-71]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin [ Nat Commun, 2025, 16(1):4491]	PubMed: 40368919
Genome rearrangements induced by the stimulation of end-joining of DNA double strand breaks through multiple phosphorylation of MRE11 by the kinase PKB/AKT1 [ Nucleic Acids Res, 2025, 53(11)gkaf468]	PubMed: 40479710
Noncanonical inhibition of topoisomerase II alpha by oxidative stress metabolites [ Redox Biol, 2025, 80:103504]	PubMed: 39879737
PARP10 promotes the repair of nascent strand DNA gaps through RAD18 mediated translesion synthesis [ Nat Commun, 2024, 15(1):6197]	PubMed: 39043663
The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex [ Mol Cell, 2024, S1097-2765(24)00285-5]	PubMed: 38703770
Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair [ Nucleic Acids Res, 2024, gkae721]	PubMed: 39180395
CAF-1 promotes efficient PrimPol recruitment to nascent DNA for single-stranded DNA gap formation [ Nucleic Acids Res, 2024, gkae1068]	PubMed: 39558157
SNF2L suppresses nascent DNA gap formation to promote DNA synthesis [ Nucleic Acids Res, 2024, gkae903]	PubMed: 39413208
Schlafen 11 further sensitizes BRCA-deficient cells to PARP inhibitors through single-strand DNA gap accumulation behind replication forks [ Oncogene, 2024, 43(32):2475-2489]	PubMed: 38961202
RHOJ controls EMT-associated resistance to chemotherapy [ Nature, 2023, 616(7955):168-175]	PubMed: 36949199

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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