MitoQ (Mitoquinone) Mesylate

製品コードS8978 バッチS897802

印刷

化学情報

Chemical Structure Synonyms MitoQ10 mesylate, Mitoubiquinone mesylate Storage
(From the date of receipt)		 2 years  -20°C  liquid
化学式

C38H47O7PS
分子量 678.81 CAS No. 845959-50-4
Solubility (25°C)* 体外 DMSO 50 mg/mL (73.65 mM)
Water 10 mg/mL (14.73 mM)
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
2%DMSO 40%PEG300 5%TWEEN80 53%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 0.500mg/ml (0.74mM) Taking the 1 mL working solution as an example, add 20 μL of 25 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 530 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 MitoQ (Mitoquinone) mesylate is a TPP-based, mitochondria-targeted antioxidant that blocks H2O2-induced intracellular ROS responses and protects against oxidative damage. This product is a waxy solid.
in vitro

Mitoquinone blocks H2O2-induced intracellular ROS responses inmurine pancreatic acinar cells. Mitoquinone does not reduce mitochondrial depolarisation induced by either cholecystokinin (CCK) or bile acid TLCS. Mitoquinone increases basal and CCK-induced cell death in a plate-reader assay.[1]
in vivo

In a TLCS-induced AP model Mitoquinone treatment is not protective. In AP induced by caerulein hyperstimulation (CER-AP), Mitoquinone exerts mixed effects. Thus, partial amelioration of histopathology scores is observed but without reduction of the biochemical markers pancreatic trypsin or serumamylase. Lungmyeloperoxidase and interleukin-6 are concurrently increased by Mitoquinone in CER-AP. Mitoquinone causes biphasic effects on ROS production in isolated polymorphonuclear leukocytes, inhibiting an acute increase but elevating later levels.[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Pancreatic Acinar Cells
濃度 1 μM, 10 μM
反応時間 200 s, 400 s, 600 s, 800 s, 1000 s
実験の流れ

The cells are loaded with 40 nM TMRM for 30 minutes prior incubation with either 1 μM or 10 μM of MitoQ or dTPP. Cholecystokinin-8 (CCK-8, 10 nM) or bile acid taurolithocholic acid 3-sulphate (TLCS, 500 μM) is used to induce ΔΨm depolarisation. At the end of the perfusion, the protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 10 μM) is added to induce complete depolarisation of ΔΨm. The fluorescence of TMRM is excited at 543 nm and the emission is collected at 560–650 nm.
動物実験 動物モデル C57BL/6J mice
投薬量 10 mg/kg, 25 mg/kg
投与方法 IP

参考

  • https://pubmed.ncbi.nlm.nih.gov/25878403/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Neutrophil extracellular traps promote pre-metastatic niche formation in the omentum by expanding innate-like B cells that express IL-10 [ Cancer Cell, 2025, 43(1):69-85.e11] PubMed: 39753138
Mitochondrial ROS drive foam cell formation via STAT5 signaling in atherosclerosis [ Sci Adv, 2025, 11(35):eadw9952] PubMed: 40864703
IFN-α Induces Heterogenous ROS Production in Human β-Cells [ bioRxiv, 2025, 2025.02.19.639120] PubMed: 40027743
Inhibition of the mPTP and Lipid Peroxidation Is Additively Protective Against I/R Injury [ Circ Res, 2024, 134(10):1292-1305] PubMed: 38618716
Inhibition of the mPTP and Lipid Peroxidation Is Additively Protective Against I/R Injury [ Circ Res, 2024, 134(10):1292-1305] PubMed: 38618716
Acidic preconditioning induced intracellular acid adaptation to protect renal injury via dynamic phosphorylation of focal adhesion kinase-dependent activation of sodium hydrogen exchanger 1 [ Cell Commun Signal, 2024, 22(1):393] PubMed: 39118129
Acidic preconditioning induced intracellular acid adaptation to protect renal injury via dynamic phosphorylation of focal adhesion kinase-dependent activation of sodium hydrogen exchanger 1 [ Cell Commun Signal, 2024, 22(1):393.] PubMed: 39118129
Tert promotes cardiac regenerative repair after MI through alleviating ROS-induced DNA damage response in cardiomyocyte [ Cell Death Discov, 2024, 10(1):381] PubMed: 39187478
Anticancer Effects of Mitoquinone via Cell Cycle Arrest and Apoptosis in Canine Mammary Gland Tumor Cells [ Int J Mol Sci, 2024, 25(9)4923] PubMed: 38732133
SBDS Gene Mutation Increases ROS Production and Causes DNA Damage as Well as Oxidation of Mitochondrial Membranes in the Murine Myeloid Cell Line 32Dcl3 [ Biol Pharm Bull, 2024, 47(7):1376-1382] PubMed: 39085077

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。