MitoQ (Mitoquinone) mesylate

製品コードS8978 バッチS897803

印刷

化学情報

Synonyms

MitoQ10 mesylate, Mitoubiquinone mesylate

Storage
(From the date of receipt)

2 years -20°C liquid

化学式

C₃₈H₄₇O₇PS

分子量

678.81

CAS No.

845959-50-4

Solubility (25°C)*

体外

DMSO

50 mg/mL (73.65 mM)

Water

9 mg/mL (13.25 mM)

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O

2.0mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	MitoQ (Mitoquinone) mesylate is a TPP-based, mitochondria-targeted antioxidant that blocks H₂O₂-induced intracellular ROS responses and protects against oxidative damage. This product is a waxy solid.
in vitro	Mitoquinone blocks H2O2-induced intracellular ROS responses inmurine pancreatic acinar cells. Mitoquinone does not reduce mitochondrial depolarisation induced by either cholecystokinin (CCK) or bile acid TLCS. Mitoquinone increases basal and CCK-induced cell death in a plate-reader assay.^[1]
in vivo	In a TLCS-induced AP model Mitoquinone treatment is not protective. In AP induced by caerulein hyperstimulation (CER-AP), Mitoquinone exerts mixed effects. Thus, partial amelioration of histopathology scores is observed but without reduction of the biochemical markers pancreatic trypsin or serumamylase. Lungmyeloperoxidase and interleukin-6 are concurrently increased by Mitoquinone in CER-AP. Mitoquinone causes biphasic effects on ROS production in isolated polymorphonuclear leukocytes, inhibiting an acute increase but elevating later levels.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Pancreatic Acinar Cells
	濃度	1 μM, 10 μM
	反応時間	200 s, 400 s, 600 s, 800 s, 1000 s
	実験の流れ	The cells are loaded with 40 nM TMRM for 30 minutes prior incubation with either 1 μM or 10 μM of MitoQ or dTPP. Cholecystokinin-8 (CCK-8, 10 nM) or bile acid taurolithocholic acid 3-sulphate (TLCS, 500 μM) is used to induce ΔΨm depolarisation. At the end of the perfusion, the protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 10 μM) is added to induce complete depolarisation of ΔΨm. The fluorescence of TMRM is excited at 543 nm and the emission is collected at 560–650 nm.
動物実験	動物モデル	C57BL/6J mice
	投薬量	10 mg/kg, 25 mg/kg
	投与方法	IP

参考

[1] Wei Huang, et al. Mediators Inflamm. 2015;2015:901780.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Local production of reactive oxygen species drives vincristine-induced axon degeneration [ Cell Death Dis, 2023, 14(12):807]	PubMed: 38065950
O-GlcNAcylation Is Required for the Survival of Cerebellar Purkinje Cells by Inhibiting ROS Generation [ Antioxidants (Basel), 2023, 12(4)806]	PubMed: 37107182
Mitoquinone alleviates osteoarthritis progress by activating the NRF2-Parkin axis [ iScience, 2023, 26(9):107647]	PubMed: 37694150
MitoQ Protects Ovarian Organoids against Oxidative Stress during Oogenesis and Folliculogenesis In Vitro [ Int J Mol Sci, 2023, 24(2)924]	PubMed: 36674435
Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling [ Immunity, 2022, S1074-7613(22)00280-1]	PubMed: 35835107
Very-low-density lipoprotein receptor-enhanced lipid metabolism in pancreatic stellate cells promotes pancreatic fibrosis [ Immunity, 2022, 55(7):1185-1199.e8]	PubMed: 35738281
HDAC class I inhibitor domatinostat sensitizes pancreatic cancer to chemotherapy by targeting cancer stem cell compartment via FOXM1 modulation [ J Exp Clin Cancer Res, 2022, 41(1):83]	PubMed: 35241126
Benchmarking AlphaFold-enabled molecular docking predictions for antibiotic discovery [ Mol Syst Biol, 2022, 18(9):e11081]	PubMed: 36065847
PARP-inhibition reprograms macrophages toward an anti-tumor phenotype [ Cell Rep, 2022, 41-2:111462]	PubMed: 36223740
Loss of legumain induces premature senescence and mediates aging-related renal fibrosis [ Aging Cell, 2022, 21(3):e13574]	PubMed: 35195326

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。