ML-SA1

製品コードS9926 バッチS992601

印刷

化学情報

Synonyms

Mucolipin synthetic agonist 1

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₂H₂₂N₂O₃

分子量

362.42

CAS No.

332382-54-4

Solubility (25°C)*

体外

DMSO

18 mg/mL (49.66 mM)

Ethanol

2 mg/mL (5.51 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.900mg/ml (2.48mM)	Taking the 1 mL working solution as an example, add 50 μL of 18 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.900mg/ml (2.48mM)	Taking the 1 mL working solution as an example, add 50 μL of 18 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	ML-SA1 (Mucolipin synthetic agonist 1) is an activator of TRPML channels. ML-SA1 also inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 values of ML-SA1 against DENV2 RNA and ZIKV RNA are 8.93 μM and 52.99 μM, respectively. ML-SA1 induces autophagy. ML-SA1 can be used for the research of broad-spectrum antiviral.
in vitro	ML-SA1, as a potent inhibitors of DENV2 and ZIKV, can inhibit these viruses by promoting lysosome acidification and protease activity to cause viral degradation.^[2]
in vivo	Administration of ML-SA1 before transient global ischemia ameliorated neurological dysfunction possibly through the promotion of autophagy and the inhibition of apoptosis.^[3]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	A549 cells, Huh7 cells
	濃度	25 μM
	反応時間	0-14 h
	実験の流れ	A549 or Huh7 cells are seeded into 96-well plates at a density of 8 × 10<sup>3</sup> for 12 h, followed by incubation with various concentrations of eML-SA1. After 48 h of incubation, the cell are used for CCK-8 assay. A549 or Huh7 cells are seeded into 12-well plates at a density of 2 × 105 for 12 h and pretreated with 25 μM this compound for 24 h using DMSO as a control. Then, cells are incubated with 1 μM Lyso-Sensor Green DND-189 for 1 h at 37℃ and digested with 0.25% trypsin to generate a single cell suspension, which is used for Flow Cytometer to investigate the effect of this chemical on lysosomal acidification.
動物実験	動物モデル	Transient global ischemia model mice (male; C57BL/6)
	投薬量	0.3 μl/min
	投与方法	i.c.

参考

https://pubmed.ncbi.nlm.nih.gov/22415822/
https://pubmed.ncbi.nlm.nih.gov/32858116/
https://pubmed.ncbi.nlm.nih.gov/30662583/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Lysosomal Ca2+ release-facilitated TFEB nuclear translocation alleviates ischemic brain injury by attenuating autophagic/lysosomal dysfunction in neurons [ Sci Rep, 2024, 14(1):24836]	PubMed: 39438678

Lysosomal Ca2+ release-facilitated TFEB nuclear translocation alleviates ischemic brain injury by attenuating autophagic/lysosomal dysfunction in neurons [ Sci Rep, 2024, 14(1):24836]

PubMed: 39438678

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。