ML167

製品コードS7509 バッチS750901

化学情報

	Synonyms	CID 44968231	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₉H₁₇N₃O₃
	分子量	335.36	CAS No.	1285702-20-6
Solubility (25°C)*	体外	DMSO	67 mg/mL (199.78 mM)
		Ethanol	13 mg/mL warmed with 50ºC water bath (38.76 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	ML167 (CID 44968231) is a highly selective Cdc2-like kinase 4 (Clk4) inhibitor with IC50 of 136 nM, >10-fold selectivity for closely related kinases Clk1-3 and Dyrk1A/1B.
in vitro	ML167, as a probe of Clk4, shows high cell permeability in a Caco-2 assay. ^[1]

プロトコル(参考用のみ)

キナーゼアッセイ	Bioluminescent assay
キナーゼアッセイ	Two bioluminescent assays are used for the Clk4 assay. Measurement of ATP depletion is assessed by using the Kinase-Glo™ assay system, where a firefly luciferase detection reagent containing D-luciferin and buffer components are added to detect the remaining ATP, following the Clk4 kinase assay. The second system, ADP-Glo® measures kinase activity by quantifying the amount of ADP formed after the kinase reaction. Bioluminescent detection of ADP levels is achieved through the addition two different detection reagents. First, a reagent that stops the protein kinase reaction and depletes the remaining ATP is added. Then, a second reagent is added to stop ATP degradation. In addition, the second reagent also contains an enzyme, such as pyruvate kinase, that efficiently converts the ADP to ATP and the same firefly luciferase/D-luciferin components present in Kinase-Glo, which generate the luminescent signal proportional to the ADP concentration produced. Therefore, the two assay formats show opposite luminescent signal changes in response to protein kinase inhibitors.

参考

[1] Rosenthal AS, et al. Probe Reports from the NIH Molecular Libraries Program. 2010.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Application of Integrated Drug Screening/Kinome Analysis to Identify Inhibitors of Gemcitabine-Resistant Pancreatic Cancer Cell Growth. [ SLAS Discov, 2018, 23(8):850-861]	PubMed: 29742358

Application of Integrated Drug Screening/Kinome Analysis to Identify Inhibitors of Gemcitabine-Resistant Pancreatic Cancer Cell Growth. [ SLAS Discov, 2018, 23(8):850-861]

PubMed: 29742358

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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