ML 210

製品コードS0788 バッチS078801

印刷

化学情報

Chemical Structure Synonyms CID 49766530 Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C22H20Cl2N4O4
分子量 475.32 CAS No. 1360705-96-9
Solubility (25°C)* 体外 DMSO 15 mg/mL (31.55 mM)
Ethanol 4 mg/mL (8.41 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 ML-210 (CID 49766530) is a selective covalent inhibitor of cellular glutathione peroxidase 4 (GPX4) with EC50 of 0.04 μM and induces ferroptosis. ML-210 can selectively kill cells induced to express mutant RAS. ML-210 exhibits anti-cancer activity.
in vitro

ML-210 has IC50s of 71 nM, 272 nM and 107nM for BJeLR (HRASV12), BJeH-LT (without HRASV12) and DRD cell lines, respectively[2].[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 BJeLR, BJeH-LT, BJeH, DRD cell lines
濃度
反応時間 48 h
実験の流れ

36 µL of cell suspension at 28,000 cells per well are added to the assay plates (1000 cells/well), and 4 µL of the medium containing ML 210 are added to the cells. The cells are incubated for 48 hours. To measure viability, 10 µL Alamar Blue solution (50% in growth medium) is added to each well. The cells are incubated for 16 hours, and fluorescence intensity is read.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Mutant p53 protects triple-negative breast adenocarcinomas from ferroptosis in vivo [ Sci Adv, 2024, 10(7):eadk1835] PubMed: 38354236
Accumulated cholesterol protects tumours from elevated lipid peroxidation in the microenvironment [ Redox Biol, 2023, 62:102678] PubMed: 36940607
Accumulated cholesterol protects tumours from elevated lipid peroxidation in the microenvironment [ Redox Biology, 2023, Volume 62] PubMed: None
Irreversible HER2 inhibitors overcome resistance to the RSL3 ferroptosis inducer in non-HER2 amplified luminal breast cancer [ Cell Death Dis, 2023, 14(8):532] PubMed: 37596261
Prognostic and therapeutic significance of a novel ferroptosis related signature in colorectal cancer patients [ Bioengineered, 2022, 13(2):2498-2512] PubMed: 35067161
ATF6α promotes prostate cancer progression by enhancing PLA2G4A-mediated arachidonic acid metabolism and protecting tumor cells against ferroptosis [ Prostate, 2022, 10.1002/pros.24308] PubMed: 35089606
Computational analysis of cholangiocarcinoma phosphoproteomes identifies patient-specific drug targets [ Cancer Res, 2021, canres.0955.2021] PubMed: 34551960
Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers [ Nat Chem Biol, 2020, 10.1038/s41589-020-0613-y] PubMed: 32778843
Cathepsin B is a mediator of organelle-specific initiation of ferroptosis [ Biochem Biophys Res Commun, 2020, 533(4):1464-1469] PubMed: 33268027

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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