MPP+ iodide

製品コードS6301 バッチS630102

印刷

化学情報

Synonyms

N-Methyl-4-Phenylpyridinium Iodide

Storage
(From the date of receipt)

3 years-20°C(in the dark) powder

化学式

C₁₂H₁₂IN

分子量

297.13

CAS No.

36913-39-0

Solubility (25°C)*

体外

DMSO

59 mg/mL (198.56 mM)

Ethanol

20 mg/mL (67.31 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5% DMSO 1 95% Corn oil	0.295mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 5.9 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	2.9mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 58 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide), the metabolite of a neurotoxin MPTP, causes symptom of Parkinson's disease (PD) in animal models by selectively destroying dopaminergic neurons in substantia nigra. MPP+ induces autophagic cell death in SH-SY5Y cells. MPP+ induces dopamine transporter (DAT) externalization in dopaminergic (DA) neurons, but internalization of serotonin transporter (SERT) in serotonergic (5-HT) neurons.
in vitro	Vacuolin-1 potently and reversibly inhibits the fusion between autophagosomes and lysosomes in mammalian cells, thereby inducing the accumulation of autophagosomes. Vacuolin-1 activates RAB5A to block autophagosome-lysosome fusion. ^[1]
in vivo	MPP+ iodide decreases mitochondrial oxidation-reduction activity and membrane potential in rat striatum.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HeLa cells
	濃度	1 μM
	反応時間	10 min, 2 h
	実験の流れ	The active RAB5A in cells treated with vacuoin-1 or transfected with RAB5A-CA or RAB5A-DN are examined by a GST-tagged RABEP1 affinity isolation assay.
動物実験	動物モデル	Male Wistar rats
	投薬量	40 nM
	投与方法	intrastriatal infusion

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Myeloid-specific blockade of notch signaling alleviates dopaminergic neurodegeneration in Parkinson's disease by dominantly regulating resident microglia activation through NF-κB signaling [ Front Immunol, 2023, 14:1193081]	PubMed: 37680624
Myeloid-specific blockade of notch signaling alleviates dopaminergic neurodegeneration in Parkinson's disease by dominantly regulating resident microglia activation through NF-κB signaling [ Front Immunol, 2023, 14:1193081]	PubMed: 37680624

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。