MSAB

製品コードS6901 バッチS690101

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C15H15NO4S
分子量 305.35 CAS No. 173436-66-3
Solubility (25°C)* 体外 DMSO 61 mg/mL (199.77 mM)
Ethanol 10 mg/mL (32.74 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 MSAB is a selective inhibitor of Wnt/β-catenin signaling that binds to β-catenin, promoting its degradation, and specifically downregulates Wnt/β-catenin target genes. MSAB shows potent anti-tumor effects.
in vitro

MSAB impedes RAW264.7 cells and human PMBCs differentiating into OCs. MSAB decreases the expression of β‐catenin in RAW264.7 and ARP1 WT cells.[2]
in vivo

MSAB (10-20 mg/kg; i.p. daily for 2 weeks) inhibits tumor growth of Wnt-dependent cancer cells in xenograft mice and MMTV-Wnt1 transgenic mice.[3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human blood mononuclear cells (PBMCs), Human MM cell lines, HEK293, RAW264.7 cells
濃度 0, 2.5, 5, 10, 20, 40 μM
反応時間 6 days, 15 days
実験の流れ

RAW264 cells are seeded in a 24‐well plate at the density of 3,000 cells/well. Humen PBMCs cells are seeded in a 24‐well plate at the density of 1 × 106 cells/well. After 6 days (RAW264 cells) or 15 days (PBMCs), the degree of osteoclast differentiation is determined by detecting the TRAP activity of the cells.
動物実験 動物モデル Athymic nude mice (5-6 weeks) bearing HCT116, HT115, H23, or H460 cells
投薬量 10mg/kg, 20 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Hyperoxia exposure upregulates Dvl-1 and activates Wnt/β-catenin signaling pathway in newborn rat lung [ BMC Mol Cell Biol, 2023, 24(1):4] PubMed: 36726071
Inhibition of beta-catenin shows therapeutic potential in head and neck squamous cell carcinoma in vitro [ Eur Arch Otorhinolaryngol, 2023, 280(1):399-408] PubMed: 36001136
Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication [ Clin Transl Med, 2022, 12(2):e684] PubMed: 35184390
Empagliflozin activates Wnt/β-catenin to stimulate FUNDC1-dependent mitochondrial quality surveillance against type-3 cardiorenal syndrome [ Mol Metab, 2022, 64:101553] PubMed: 35863636
G-protein-coupled receptor 124 promotes osteogenic differentiation of BMSCs through the Wnt/β-catenin pathway [ In Vitro Cell Dev Biol Anim, 2022, 10.1007/s11626-022-00684-9] PubMed: 35916978
Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes [ Front Cell Dev Biol, 2021, 9:648201] PubMed: 33748142

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。