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Synonyms | Storage (From the date of receipt) |
3 years -20°C powder | ||
化学式 | C19H32N4O11 |
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分子量 | 492.48 | CAS No. | 53678-77-6 | |
Solubility (25°C)* | 体外 | DMSO | 98 mg/mL (198.99 mM) | |
Water | 98 mg/mL (198.99 mM) | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Muramyl dipeptide (MDP) is a shared structural unit of peptidoglycans. Muramyl dipeptide is an inducer of bone formation through the induction of Runx2. |
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in vitro | MDP directly augments osteoblast differentiation and bone-forming gene expression by Runx2 activation. Despite no direct effect, MDP indirectly attenuates osteoclast differentiation through down-regulation of the RANKL/OPG ratio. MDP increases the expression of the MDP receptor, Nod2, and MDP-induced bone formation and osteoblast activation does not occur during Nod2-deficiency.[1] |
in vivo | Increased bone and mineral density by enhanced bone formation are observed in mice administered with Muramyl dipeptide (MDP). Remarkably, pre-treatment or post-treatment with MDP alleviates bone loss in RANKL-induced osteoporosis mouse models.[1] |
細胞アッセイ | 細胞株 | MC3T3-E1 cells, BMSCs, or primary osteoblast precursors from mouse calvaria |
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濃度 | 0.1 μg/ml, 1 μg/ml, 10 μg/ml | |
反応時間 | 2 days | |
実験の流れ | MC3T3-E1 cells, BMSCs, or primary osteoblast precursors from mouse calvaria are plated onto 48-well plates at 2×104 cells/400 μl/well and incubated with osteoblast induction medium in the absence or presence of MDP. Half of the medium is replaced with fresh osteoblast induction medium every 2 days. At day 6 and 12, the cells are stained with ALP for osteoblast differentiation and with alizarin red S for bone mineralization, respectively. |
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動物実験 | 動物モデル | Five-week-old C57BL/6 mice, one-day-old neonatal mice, B6.129S1-Nod2tm1Flv/J mice |
投薬量 | 1.25 mg/kg | |
投与方法 | IP |
Autophagy in the HTR-8/SVneo Cell Oxidative Stress Model Is Associated with the NLRP1 Inflammasome [ Oxid Med Cell Longev, 2021, 2021:2353504] | PubMed: 33854691 |
The Ferroptosis-NLRP1 Inflammasome: The Vicious Cycle of an Adverse Pregnancy [ Front Cell Dev Biol, 2021, 9:707959] | PubMed: 34490257 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。