Muramyl dipeptide (MDP)

製品コードS9709 バッチS970901

印刷

化学情報

Synonyms

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₉H₃₂N₄O₁₁

分子量

492.48

CAS No.

53678-77-6

Solubility (25°C)*

体外

DMSO

98 mg/mL (198.99 mM)

Water

98 mg/mL (198.99 mM)

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	4.900mg/ml (9.95mM)	Taking the 1 mL working solution as an example, add 50 μL of 98 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.225mg/ml (2.49mM)	Taking the 1 mL working solution as an example, add 50 μL of 24.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Muramyl dipeptide (MDP) is a shared structural unit of peptidoglycans. Muramyl dipeptide is an inducer of bone formation through the induction of Runx2.
in vitro	Muramyl dipeptide (MDP) directly augments osteoblast differentiation and bone-forming gene expression by Runx2 activation. Despite no direct effect, it indirectly attenuates osteoclast differentiation through down-regulation of the RANKL/OPG ratio. This compound increases the expression of its receptor, Nod2, and MDP-induced bone formation and osteoblast activation does not occur during Nod2-deficiency.^[1]
in vivo	Increased bone and mineral density by enhanced bone formation are observed in mice administered with Muramyl dipeptide (MDP). Remarkably, pre-treatment or post-treatment with this compound alleviates bone loss in RANKL-induced osteoporosis mouse models.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	MC3T3-E1 cells, BMSCs, or primary osteoblast precursors from mouse calvaria
	濃度	0.1 μg/ml, 1 μg/ml, 10 μg/ml
	反応時間	2 days
	実験の流れ	MC3T3-E1 cells, BMSCs, or primary osteoblast precursors from mouse calvaria are plated onto 48-well plates at 2×10⁴ cells/400 μl/well and incubated with osteoblast induction medium in the absence or presence of Muramyl dipeptide (MDP). Half of the medium is replaced with fresh osteoblast induction medium every 2 days. At day 6 and 12, the cells are stained with ALP for osteoblast differentiation and with alizarin red S for bone mineralization, respectively.
動物実験	動物モデル	Five-week-old C57BL/6 mice, one-day-old neonatal mice, B6.129S1-Nod2^tm1Flv/J mice
	投薬量	1.25 mg/kg
	投与方法	IP

参考

https://pubmed.ncbi.nlm.nih.gov/28337794/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Autophagy in the HTR-8/SVneo Cell Oxidative Stress Model Is Associated with the NLRP1 Inflammasome [ Oxid Med Cell Longev, 2021, 2021:2353504]	PubMed: 33854691
The Ferroptosis-NLRP1 Inflammasome: The Vicious Cycle of an Adverse Pregnancy [ Front Cell Dev Biol, 2021, 9:707959]	PubMed: 34490257

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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