Datasheet

生物学的記述

Specificity	Myc tag Antibody [A14H15] detects recombinant proteins containing the Myc epitope tag.
Background	The Myc tag is a widely used small epitope tag consisting of an 11-amino-acid peptide sequence (MEQKLISEEDL) derived from the c-Myc protein, belonging to the family of epitope tags used for labeling and detecting recombinant proteins across various expression systems, including bacteria, yeast, insect, and mammalian cells. The Myc tag is compact and hydrophilic, which minimizes interference with the native structure and function of the fused protein. It is genetically fused to target proteins via recombinant DNA technology, allowing easy detection with highly specific monoclonal antibodies. It enables diverse applications such as immunoblotting (Western blotting) to detect protein expression and size, immunoprecipitation for isolating protein complexes, immunostaining for visualizing localization in cells, and flow cytometry for quantification. Its small size (<2 kDa) ensures minimal perturbation of the target protein. The Myc tag can also be combined with other tags, enhancing purification and interaction studies, thus simplifying protein characterization and accelerating research in cellular and molecular biology.

Specificity

Myc tag Antibody [A14H15] detects recombinant proteins containing the Myc epitope tag.

Background

The Myc tag is a widely used small epitope tag consisting of an 11-amino-acid peptide sequence (MEQKLISEEDL) derived from the c-Myc protein, belonging to the family of epitope tags used for labeling and detecting recombinant proteins across various expression systems, including bacteria, yeast, insect, and mammalian cells. The Myc tag is compact and hydrophilic, which minimizes interference with the native structure and function of the fused protein. It is genetically fused to target proteins via recombinant DNA technology, allowing easy detection with highly specific monoclonal antibodies. It enables diverse applications such as immunoblotting (Western blotting) to detect protein expression and size, immunoprecipitation for isolating protein complexes, immunostaining for visualizing localization in cells, and flow cytometry for quantification. Its small size (<2 kDa) ensures minimal perturbation of the target protein. The Myc tag can also be combined with other tags, enhancing purification and interaction studies, thus simplifying protein characterization and accelerating research in cellular and molecular biology.

使用情報

Application

WB, IP. IHC, IF, FCM

Dilution

WB	IF	FCM
1:500 - 1:1000	1:400	1:2000

Reactivity

Source

Mouse Monoclonal Antibody

MW

48 kDa,49 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/40537033/
https://pubmed.ncbi.nlm.nih.gov/31992583/

Application Data

WB

Validated by Selleck

Lane 1: HEK-293T, Lane 2: HEK-293T (MYC-GFP transfected)