MYF-01-37

製品コードS8950 バッチS895001

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₅H₁₇F₃N₂O
	分子量	298.3	CAS No.	2416417-65-5
Solubility (25°C)*	体外	DMSO	60 mg/mL (201.13 mM)
		Ethanol	60 mg/mL (201.13 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	MYF-01-37 is a novel covalent inhibitor of TEAD targeting Cys380 when incubated with the TEAD2 protein.
in vitro	MYF-01-37 is developed as a covalent binder to TEAD, targeting Cys380 when incubated with the TEAD2 protein (C359 in TEAD1). Pretreating cells with MYF-01-37 leads to loss of direct TEAD pull-down by biotin-MYF-01-037 from whole-cell lysate, confirming MYF-01-037 binding to TEAD in cells.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	PC9 cells
	濃度	10 μM, 1 μM
	反応時間	6 h
	実験の流れ	Cells are treated with test compounds for 6 h at the indicated doses. Total cell lysates are prepared using pulldown buffer. To pull down TEADs, 1 mg of total protein is combined with biotinylated MYF-01-37 at 1 μM and rotated at 4 ℃ for 6 hrs followed by RT for 1 h. Subsequently, streptavidin agarose resin (30 ml 50% slurry) is added and samples rotated for another 2 h at 4 ℃. The resin is subsequently washed 3× with pulldown buffer, and TEADs released from the resin by boiling for 10 min in 2× gel loading buffer and resolved by western blotting. As a loading control, 25 mg of total protein is used.

参考

[1] Kari J Kurppa, et al. Cancer Cell. 2020 Jan 13;37(1):104-122.e12.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Targeting the Hippo/YAP/TAZ signalling pathway: Novel opportunities for therapeutic interventions into skin cancers [ Exp Dermatol, 2022, 31(10):1477-1499]	PubMed: 35913427

Targeting the Hippo/YAP/TAZ signalling pathway: Novel opportunities for therapeutic interventions into skin cancers [ Exp Dermatol, 2022, 31(10):1477-1499]

PubMed: 35913427

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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