MYF-01-37

製品コードS8950 バッチS895002

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₅H₁₇F₃N₂O

分子量

298.3

CAS No.

2416417-65-5

Solubility (25°C)*

体外

DMSO

60 mg/mL (201.13 mM)

Ethanol

60 mg/mL (201.13 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	3.000mg/ml (10.06mM)	Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.500mg/ml (5.03mM)	Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	MYF-01-37 is a novel covalent inhibitor of TEAD targeting Cys380 when incubated with the TEAD2 protein.
in vitro	MYF-01-37 is developed as a covalent binder to TEAD, targeting Cys380 when incubated with the TEAD2 protein (C359 in TEAD1). Pretreating cells with this compound leads to loss of direct TEAD pull-down by biotin-MYF-01-037 from whole-cell lysate, confirming this chemical binding to TEAD in cells.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	PC9 cells
	濃度	10 μM, 1 μM
	反応時間	6 h
	実験の流れ	Cells are treated with test compounds for 6 h at the indicated doses. Total cell lysates are prepared using pulldown buffer. To pull down TEADs, 1 mg of total protein is combined with biotinylated MYF-01-37 at 1 μM and rotated at 4 ℃ for 6 hrs followed by RT for 1 h. Subsequently, streptavidin agarose resin (30 ml 50% slurry) is added and samples rotated for another 2 h at 4 ℃. The resin is subsequently washed 3× with pulldown buffer, and TEADs released from the resin by boiling for 10 min in 2× gel loading buffer and resolved by western blotting. As a loading control, 25 mg of total protein is used.

参考

https://pubmed.ncbi.nlm.nih.gov/31935369/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Targeting the Hippo/YAP/TAZ signalling pathway: Novel opportunities for therapeutic interventions into skin cancers [ Exp Dermatol, 2022, 31(10):1477-1499]	PubMed: 35913427

Targeting the Hippo/YAP/TAZ signalling pathway: Novel opportunities for therapeutic interventions into skin cancers [ Exp Dermatol, 2022, 31(10):1477-1499]

PubMed: 35913427

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。