NEM (N-Ethylmaleimide)

製品コードS3692 バッチS369204

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C6H7NO2
分子量 125.13 CAS No. 128-53-0
Solubility (25°C)* 体外 DMSO 25 mg/mL (199.79 mM)
Water 25 mg/mL (199.79 mM)
Ethanol 25 mg/mL (199.79 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 NEM (N-Ethylmaleimide) is an organic compound that is derived from maleic acid. It is an irreversible inhibitor of all cysteine peptidases, with alkylation occurring at the active site thiol group. N-Ethylmaleimide (NEM) inactivates endogenous deubiquitinating enzymes (DUBs). N-Ethylmaleimide (NEM) specifically inhibits phosphate transport in mitochondria.
in vitro

N-Ethylmaleimide inactivates endogenous deubiquitinating enzymes (DUBs) and specifically inhibits phosphate transport in mitochondria.[1]
in vivo

N-Ethylmaleimide is an irreversible cysteine protease (sulfhydryl) inhibitor.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HEK293T cells
濃度 10 mM
反応時間 14 h
実験の流れ

For endogenous protein immunoprecipitation, cell lysates are prepared in Pierce IP Lysis Buffer containing protease inhibitors, phosphatase inhibitor and deubiquitinating inhibitor N-Ethylmaleimide (NEM, 10mM). Lysate supernatant is firstly incubated with primary antibody at 4 ℃ for 14 h, and then mixed with Dynabeads Protein G magnetic beads at 4 ℃ for 4 h on a rotator. The magnetic bead-Ab complex is separated by Magnetic Separation Rack and the beads are washed three times with PBST buffer. For SDS-PAGE analysis, the complex-bound beads are resuspended in 1x protein electrophoresis loading buffer and boiled at 100 ℃ for 8 min before the supernatant is collected. For exogenous protein immunoprecipitation, cells are transfected for 48 h before preparation of cell lysates. To prepare negative control, antibody against mouse IgG is used in parallel.
動物実験 動物モデル Male Sprague-Dawley rats
投薬量 10 mg/kg
投与方法 s.c.

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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