N-Succinimidyl-S-acetylthioacetate (SATA)

製品コードS0057 バッチS005701

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₈H₉NO₅S

分子量

231.23

CAS No.

76931-93-6

Solubility (25°C)*

体外

DMSO

46 mg/mL (198.93 mM)

Ethanol

4 mg/mL (17.29 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	N-Succinimidyl-S-acetylthioacetate (SATA) is a protein modification agent which introduces thiol-groups into protein molecules. N-Succinimidyl-S-acetylthioacetate (SATA) adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form.
in vitro	SATA allows the introduction of sulfhydryl groups into proteins by the reaction of its active NHS ester end with amino groups, without affecting the indigenous disulfides of the protein.^[2]
in vivo	Due to antibody degradation, and spontaneous precipitation of the SATA conjugated antibody upon storage, this compound is not a suitable bifunctional chelating agent (BCA).^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	A-431, MRC-5 cell lines
	濃度	--
	反応時間	4 h
	実験の流れ	The conjugation procedure is performed through derivatization of nimotuzumab with N-Succinimidyl-S-acetylthioacetate (SATA) followed by a covalent attachment with maleimide groups at the end of PEG-DSPE chains located at the membrane of pre-formed liposomes. Cells are seeded at 80% confluence in chamber slides. After 24 h of culture, cells are incubated with 40 mL of a dilution 1:50 of liposomes or immunoliposomes (ILs) and then incubated for 4 h at 37 ℃. Unbound liposomes or ILs are removed by washing (3×) with PBS 1×(pH 7.4) and fixed with 3.7% paraformaldehyde. The samples are washed again with PBS 1×and mounted with vectashield mounting medium.
動物実験	動物モデル	B16-F10 tumor bearing C57BL6 mice
	投薬量	7 μL 2 mg/mL
	投与方法

参考

https://pubmed.ncbi.nlm.nih.gov/30010404/
https://pubmed.ncbi.nlm.nih.gov/27367153/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。