Narciclasine

製品コードE1124 バッチE112401

印刷

化学情報

Synonyms

Lycoricidinol

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₄H₁₃NO₇

分子量

307.26

CAS No.

29477-83-6

Solubility (25°C)*

体外

DMSO

61 mg/mL (198.52 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	3.050mg/ml (9.93mM)	Taking the 1 mL working solution as an example, add 50 μL of 61 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.510mg/ml (1.66mM)	Taking the 1 mL working solution as an example, add 50 μL of 10.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Narciclasine (Lycoricidinol), an isocarbostyril alkaloid narciclasine, targets STAT3 via distinct mechanisms in tamoxifen-resistant breast cancer cells. Narciclasine suppresses phosphorylation, activation, dimerization, and nuclear translocation of STAT3 by directly binding with the STAT3 SH2 domain and leads STAT3 degradation. Narciclasine is a plant growth modulator. Narciclasine modulates the Rho/Rho kinase/LIM kinase/cofilin signaling pathway, greatly increasing GTPase RhoA activity as well as inducing actin stress fiber formation in a RhoA-dependent manner.
in vitro	Narciclasine activates Rho and stress fibers in glioblastoma multiforme cells. The mean IC50 of ~50 nM calculated on the 6 human glioblastoma multiforme (U373, Hs683, GL19, GL5, GL16, GL17), The mean IC50 value of 47 nM for this compound across a panel of 60 cancer cell lines. ^[2]
in vivo	Narciclasine significantly increases the survival of GL19 glioblastoma multiforme-bearing mice, and the survival of Hs683 glioblastoma multiforme-bearing mice. ^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human glioblastoma multiforme cell lines
	濃度	0.001-10 μM
	反応時間	72 h
	実験の流れ	The cells are incubated for 72 h in the presence and absence of the narciclasine (0.001-10 μM) for the determination of its IC50 values by colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
動物実験	動物モデル	GL19 glioblastoma multiforme-bearing mice
	投薬量	1 mg/kg
	投与方法	i.v., p.o.

参考

https://pubmed.ncbi.nlm.nih.gov/27542176/
https://pubmed.ncbi.nlm.nih.gov/19531573/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Local soft niches in mechanically heterogeneous primary tumors promote brain metastasis via mechanotransduction-mediated HDAC3 activity [ Sci Adv, 2025, 11(9):eadq2881]	PubMed: 40009679
Cell mechanics regulate the migration and invasion of hepatocellular carcinoma cells via JNK signaling [ Acta Biomater, 2024, S1742-7061(24)00036-9]	PubMed: 38272199
The mechanics of local niches in the primary tumor regulate breast cancer brain metastasis [ POLYU ELECTRONIC THESES, 2023, viii, 164]	PubMed: None

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。