Necrostatin 2 racemate (Nec-1s)

製品コードS8641 バッチS864104

印刷

化学情報

Chemical Structure Synonyms 7-Cl-O-Nec1, Necrostatin-1 stable, Necrostatin 1S Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C13H12ClN3O2
分子量 277.71 CAS No. 852391-15-2
Solubility (25°C)* 体外 DMSO 56 mg/mL (201.64 mM)
Ethanol 18 mg/mL (64.81 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Necrostatin 2 racemate (Nec-1s, 7-Cl-O-Nec1, Necrostatin-1 stable, Necrostatin 1S) は、Necrostatin-1 の安定型変異体です。これは、IDO を標的とする効果を持たない、より特異的な RIPK1 阻害剤であり、485 種類すべてのヒトキナーゼの中で、他のキナーゼよりも RIPK1 に対して 1000 倍以上選択的です。
in vitro

Necrostatin 2 racemate (Nec-1s) is a potent inhibitor of RIPK1 and cellular necroptosis while lacking IDO inhibitory activity.
in vivo

Necrostatin 2 racemate (Nec-1s) is effective in reducing brain injuries. It is a superior inhibitor suitable for use in vivo lacking a paradoxical sensitizing effect in TNF-induced lethality.

This compound possesses several advantageous pharmacokinetic and pharmacodynamic characteristics in comparison to Nec-1. Its safety profile is also superior to that of Nec-1 for reduced in vivo and in vitro toxicity.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 BMDMs
濃度 50 µM
反応時間 6 h
実験の流れ Cells were treated with indicated concentrations of Necrostatin 2 racemate (Nec-1s).
動物実験 動物モデル TNF-induced SIRS model (Female C57BL/6J WT mice)
投薬量 0.6 mg/kg and 6 mg/kg
投与方法 i.v.

参考

  • https://pubmed.ncbi.nlm.nih.gov/23190609/
  • https://pubmed.ncbi.nlm.nih.gov/30081531/
  • https://pubmed.ncbi.nlm.nih.gov/36198273/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Iron promotes both ferroptosis and necroptosis in the early stage of reperfusion in ischemic stroke [ Neural Regeneration Research, Not available, Not available] PubMed: 39286656
The glutathione peroxidase Gpx4 prevents lipid peroxidation and ferroptosis to sustain Treg cell activation and suppression of antitumor immunity [ Cell Reports, June 15, 2021, 109235] PubMed: 34133924
Nonstructural Protein NSs Activates Inflammasome and Pyroptosis through Interaction with NLRP3 in Human Microglial Cells Infected with Severe Fever with Thrombocytopenia Syndrome Bandavirus [ Journal of Virology, June 13, 2022, Not available]
RIPK1 Regulates Microglial Activation in Lipopolysaccharide-Induced Neuroinflammation and MPTP-Induced Parkinsons Disease Mouse Models [ Cells, January 26, 2023, 417] PubMed: 36766759
Ddb1 Is Essential for the Expansion of CD4+ Helper T Cells by Regulating Cell Cycle Progression and Cell Death [ Frontiers in Immunology, August 29, 2021, 724045] PubMed: 34526995
SIGLEC12 mediates plasma membrane rupture during necroptotic cell death [ Nature, 2025, 10.1038/s41586-025-09741-1] PubMed: 41225007
Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukemia [ Cell, 2025, S0092-8674(25)01233-4] PubMed: 41265435
Radiotherapy promotes cuproptosis and synergizes with cuproptosis inducers to overcome tumor radioresistance [ Cancer Cell, 2025, S1535-6108(25)00132-1] PubMed: 40215978
Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma [ Cancer Commun (Lond), 2025, 10.1002/cac2.70036] PubMed: 40443016
MLKL‒OPTN axis regulates herpesvirus-induced neurological sequelae [ Clin Transl Med, 2025, 15(6):e70353] PubMed: 40490945

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。