Necrosulfonamide

製品コードS8251 バッチS825101

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₈H₁₅N₅O₆S₂
	分子量	461.47	CAS No.	1360614-48-7
Solubility (25°C)*	体外	DMSO	92 mg/mL (199.36 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Necrosulfonamide is a very specific and potent necrosis inhibitor and blocks mixed lineage kinase domain-like protein (MLKL).
in vitro	Necrosulfonamide inhibits MLKL-mediated Necrosis by blocking its N-terminal CC domain function. It blocks necrosis downstream of RIP3 activation. This compound has no effect on apoptosis induced by TNF-α plus Smac mimetic in non-RIP3-expressing Panc-1 cells, even at 5 μM concentration. The reason for the species specificity of this chemical is that the cysteine at residue 86 in human MLKL that is covalently modified by it is replaced by a tryptophan residue in mouse MLKL(mixed lineage kinase domain-like protein).
in vivo	Necrosulfonamide (NSA) is a small molecule that specifically inhibits necroptosis by targeting MLKL, the terminal executioner of this process.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HT-29 cells
	濃度	1 μM
	反応時間	8 or 12 hrs
	実験の流れ	Necrosis inhibitors induce diverse effects on MLKL phosphorylation. HT-29 cells are treated with T/S/Z with or without necrosis inhibitors for 12 hr or 8 hr. The number of dead cells is determined by measuring released protease activity in culture medium. The whole-cell extracts are prepared and analyzed by western blotting. The final concentrations of 10 μM necrostatin-1 or 1 μM this compound are used to block necrosis.
動物実験	動物モデル	Male Wistar rats
	投薬量	1.65 mg/kg
	投与方法	i.p.

参考

http://pubs.rsc.org/en/content/articlelanding/2014/md/c3md00278k#!divAbstract
https://pubmed.ncbi.nlm.nih.gov/22265413/
https://pubmed.ncbi.nlm.nih.gov/24703947/

https://pubmed.ncbi.nlm.nih.gov/32936609/

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カスタマーフィードバック

: Data from [Data independently produced by , , Biochim Biophys Acta, 2018, 1865(3):522-531]

: Data from [Data independently produced by , , Biochem Biophys Res Commun, 2018, 503(3):1550-1556]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

SIGLEC12 mediates plasma membrane rupture during necroptotic cell death [ Nature, 2025, 10.1038/s41586-025-09741-1]	PubMed: 41225007
STING induces ZBP1-mediated necroptosis independently of TNFR1 and FADD [ Nature, 2025, 647(8090):735-746]	PubMed: 40834903
Cytosolic cytochrome c represses ferroptosis [ Cell Metab, 2025, S1550-4131(25)00149-4]	PubMed: 40233758
Harnessing the FGFR2/NF2/YAP signaling-dependent necroptosis to develop an FGFR2/IL-8 dual blockade therapeutic strategy [ Nat Commun, 2025, 16(1):4128]	PubMed: 40319089
MLKL‒OPTN axis regulates herpesvirus-induced neurological sequelae [ Clin Transl Med, 2025, 15(6):e70353]	PubMed: 40490945
The cancer-associated SF3B1K700E spliceosome mutation confers enhanced sensitivity to BV-6-induced cytotoxicity [ Cell Death Dis, 2025, 16(1):476]	PubMed: 40595498
Androgen receptor inhibition sensitizes glioblastoma stem cells to temozolomide by the miR-1/miR-26a-1/miR-487b signature mediated WT1 and FOXA1 silencing [ Cell Death Discov, 2025, 11(1):248]	PubMed: 40399259
O-GlcNAcylation of glutaminase isoform KGA inhibits ferroptosis through activation of glutaminolysis in hepatoblastoma [ Cell Death Discov, 2025, 11(1):160]	PubMed: 40204725
Neuregulin-1 prevents death from a normally lethal respiratory viral infection [ PLoS Pathog, 2025, 21(4):e1013124]	PubMed: 40267147
WTX-L/β-arrestin2/LCN2 axis controls vulnerability to ferroptosis in gastric cancer [ iScience, 2025, 28(3):111964]	PubMed: 40109379

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。