NGI-1

製品コードS8750 バッチS875001

印刷

化学情報

Synonyms

ML414

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₇H₂₂N₄O₃S₂

分子量

394.51

CAS No.

790702-57-7

Solubility (25°C)*

体外

DMSO

79 mg/mL (200.24 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5% DMSO 1 95% Corn oil	0.49mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 9.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.95mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 79 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	NGI-1 is an aminobenzamide-sulfonamide compound that targets both oligosaccharyltransferase (OST) isoforms and therefore may exhibit antiviral activity against flaviviruses.
in vitro	NGI-1 is a reversible catalytic subunit inhibitor of the oligosaccharyltransferase (OST) that has higher specificity for STT3B compared to STT3A (STT: OST catalytic subunit). In non-small cell lung cancer cells NGI-1 blocks cell surface localization and signaling of the EGFR glycoprotein, but selectively arrests proliferation in only those cell lines that are dependent on EGFR (or FGFR) for survival. In these cell lines OST inhibition causes cell cycle arrest accompanied by induction of p21, autofluorescence, and changes in cell morphology; all hallmarks of senescence^[1]. NGI-1 exhibits broad antiviral activity against multiple flaviviruses. It blocks viral RNA replication independent of inhibition of the catalytic activity of the OST complex^[2].

製品説明

NGI-1 is an aminobenzamide-sulfonamide compound that targets both oligosaccharyltransferase (OST) isoforms and therefore may exhibit antiviral activity against flaviviruses.

in vitro

NGI-1 is a reversible catalytic subunit inhibitor of the oligosaccharyltransferase (OST) that has higher specificity for STT3B compared to STT3A (STT: OST catalytic subunit). In non-small cell lung cancer cells NGI-1 blocks cell surface localization and signaling of the EGFR glycoprotein, but selectively arrests proliferation in only those cell lines that are dependent on EGFR (or FGFR) for survival. In these cell lines OST inhibition causes cell cycle arrest accompanied by induction of p21, autofluorescence, and changes in cell morphology; all hallmarks of senescence^[1]. NGI-1 exhibits broad antiviral activity against multiple flaviviruses. It blocks viral RNA replication independent of inhibition of the catalytic activity of the OST complex^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	PC9 and A549 cells
	濃度	10 μM
	反応時間	24 h
	実験の流れ	PC9 and A549 cells were cultured in 6-well plates in serum-containing medium and treated with or without 10 μM NGI-1 for 24 hours.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

ER Stress-Activated HSF1 Governs Cancer Cell Resistance to USP7 Inhibitor-Based Chemotherapy through the PERK Pathway [ Int J Mol Sci, 2024, 25(5)2768]	PubMed: 38474017
Glycosylation of FGFR4 in cholangiocarcinoma regulates receptor processing and cancer signaling [ J Cell Biochem, 2022, 10.1002/jcb.30204]	PubMed: 34981854
Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors [ Cancer Res, 2018, 78(17):5094-5106]	PubMed: 30026325

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。