Nigericin sodium salt

製品コードS6653 バッチS665302

印刷

化学情報

Synonyms

NSC 292567

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₄₀H₆₇NaO₁₁

分子量

746.94

CAS No.

28643-80-3

Solubility (25°C)*

体外

Ethanol

65 mg/mL (87.02 mM)

DMSO

50 mg/mL (66.93 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution (main for i.p.)

5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O

2.5mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H⁺, K⁺, and Pb²⁺ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.
in vitro	In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H⁺, K⁺, and Pb²⁺ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade^[1] . Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death. ^[2]
in vivo	Nigericin is a NOD-like receptor protein 3 (NLRP3) activator.

製品説明

Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H⁺, K⁺, and Pb²⁺ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.

in vitro

In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H⁺, K⁺, and Pb²⁺ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade^[1] .

Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death. ^[2]

in vivo

Nigericin is a NOD-like receptor protein 3 (NLRP3) activator.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	H460 cells
	濃度	1 µM
	反応時間	24 h
	実験の流れ	H460 cells are seeded in 8-µm pore size Transwell® Inserts in RPMI 1640 (without FBS) with or without drugs (DMSO as control) or NIG (1µM). Inserts are placed in 24-well plates with RPMI 1640 containing 10% FBS in the presence of DMSO (control) or NIG (1 µM).
動物実験	動物モデル	Male C57BL/6 mice
	投薬量	4 mg/kg
	投与方法	i.p.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

3'-Deoxyadenosin alleviates methamphetamine-induced aberrant synaptic plasticity and seeking behavior by inhibiting the NLRP3 inflammasome [ Neural Regen Res, 2024, 19(10):2270-2280]	PubMed: 38488561
ER stress induces caspase-2-tBID-GSDME-dependent cell death in neurons lytically infected with herpes simplex virus type 2 [ EMBO J, 2023, e113118.]	PubMed: 37646198
Lipocalin-2-mediated astrocyte pyroptosis promotes neuroinflammatory injury via NLRP3 inflammasome activation in cerebral ischemia/reperfusion injury [ J Neuroinflammation, 2023, 20(1):148]	PubMed: 37353794
Stellate ganglion block ameliorated central post-stroke pain with comorbid anxiety and depression through inhibiting HIF-1α/NLRP3 signaling following thalamic hemorrhagic stroke [ J Neuroinflammation, 2023, 20(1):82]	PubMed: 36944982
A novel sorbicillinoid compound as a potent anti-inflammation agent through inducing NLRP3 protein degradation [ Br J Pharmacol, 2023, 10.1111/bph.16058]	PubMed: 36788033
Takinib inhibits microglial M1 polarization and oxidative damage after subarachnoid hemorrhage by targeting TAK1-dependent NLRP3 inflammasome signaling pathway [ Front Immunol, 2023, 14:1266315]	PubMed: 38035075
Eleutheroside E from pre-treatment of Acanthopanax senticosus (Rupr.etMaxim.) Harms ameliorates high-altitude-induced heart injury by regulating NLRP3 inflammasome-mediated pyroptosis via NLRP3/caspase-1 pathway [ Int Immunopharmacol, 2023, 121:110423]	PubMed: 37331291
Sipeimine attenuates PM2.5-induced lung toxicity via suppression of NLRP3 inflammasome-mediated pyroptosis through activation of the PI3K/AKT pathway [ Chem Biol Interact, 2023, 376:110448]	PubMed: 36898572
Electroacupuncture improves gout arthritis pain via attenuating ROS-mediated NLRP3 inflammasome overactivation [ Chin Med, 2023, 18(1):86]	PubMed: 37464384
Salidroside pretreatment alleviates PM2.5 caused lung injury via inhibition of apoptosis and pyroptosis through regulating NLRP3 Inflammasome [ Food Chem Toxicol, 2023, 177:113858]	PubMed: 37236293

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。