Nigericin sodium salt

製品コードS6653 バッチS665303

印刷

化学情報

Synonyms

NSC 292567

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₄₀H₆₇NaO₁₁

分子量

746.94

CAS No.

28643-80-3

Solubility (25°C)*

体外

Ethanol

100 mg/mL (133.87 mM)

DMSO

Insoluble

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5%absolute ethyl alcohol 1 40% PEG 300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.200mg/ml (0.27mM)	Taking the 1 mL working solution as an example, add 50 μL of 4mg/ml absolute ethyl alcohol stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%absolute ethyl alcohol 1 95% saline この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.200mg/ml (0.27mM)	Taking the 1 mL working solution as an example, add 50 μL of 4 mg/ml clear absolute ethyl alcohol stock solution to 950 μL of saline and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Nigericin sodium saltは、Streptomyces hygroscopicusに由来する抗生物質で、H⁺、K⁺、およびPb²⁺イオノフォアとして作用します。Nigericinは、NLRP3インフラマソームを活性化して、炎症誘発性および免疫刺激性プロセスを誘導します。
in vitro	In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H⁺, K⁺, and Pb²⁺ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade . Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death.
in vivo	Nigericin is a NOD-like receptor protein 3 (NLRP3) activator.

製品説明

Nigericin sodium saltは、Streptomyces hygroscopicusに由来する抗生物質で、H⁺、K⁺、およびPb²⁺イオノフォアとして作用します。Nigericinは、NLRP3インフラマソームを活性化して、炎症誘発性および免疫刺激性プロセスを誘導します。

in vitro

In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H⁺, K⁺, and Pb²⁺ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade .

Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death.

in vivo

Nigericin is a NOD-like receptor protein 3 (NLRP3) activator.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	H460 cells
	濃度	1 µM
	反応時間	24 h
	実験の流れ	H460 cells are seeded in 8-µm pore size Transwell® Inserts in RPMI 1640 (without FBS) with or without drugs (DMSO as control) or NIG (1µM). Inserts are placed in 24-well plates with RPMI 1640 containing 10% FBS in the presence of DMSO (control) or NIG (1 µM).
動物実験	動物モデル	Male C57BL/6 mice
	投薬量	4 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/28351327-nigericin-decreases-the-viability-of-multidrug-resistant-cancer-cells-and-lung-tumorspheres-and-potentiates-the-effects-of-cardiac-glycosides/?from_single_result=Nigericin+decreases+the+viability+of+multidrug-resistant+cancer+cells+and+lung+tumorspheres+and+potentiates+the+effects+of+cardiac+glycosides
https://pubmed.ncbi.nlm.nih.gov/24089192-mitochondrial-reactive-oxygen-species-induces-nlrp3-dependent-lysosomal-damage-and-inflammasome-activation/?from_single_result=Mitochondrial+ROS+induces+NLRP3-dependent+lysosomal+damage+and+inflammasome+activation
https://pubmed.ncbi.nlm.nih.gov/35462332/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

NINJ1 regulates plasma membrane fragility under mechanical strain [ Nature, 2025, 10.1038/s41586-025-09222-5]	PubMed: 40490006
Hepatocyte-Specific GSDMD Deficiency Aggravates Sepsis by Disrupting Non-Canonical Secretion of Anti-Inflammatory Factors [ Adv Sci (Weinh), 2025, 12(43):e10412]	PubMed: 40856013
PM2.5 exacerbates nasal epithelial barrier dysfunction in allergic rhinitis by inducing NLRP3-mediated pyroptosis via the AhR/CYP1A1/ROS axis [ J Hazard Mater, 2025, 492:138145]	PubMed: 40209413
ALIX mediates reversible gasdermin-D pore formation via the endosomal pathway to limit pyroptosis by active membrane repair [ Cell Death Dis, 2025, 16(1):681]	PubMed: 41053117
Targeting RPLP2 Triggers DLBCL Ferroptosis by Decreasing FXN Expression [ Biomedicines, 2025, 13(6)1320]	PubMed: 40564039
GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC [ Mediators Inflamm, 2025, 2025:5576056]	PubMed: 40862294
Human cytomegalovirus protein US18 promotes NLRP3 inflammasome activation [ Biochem Biophys Res Commun, 2025, 775:152110]	PubMed: 40450990
Small nuclear ribonucleoprotein polypeptide B2 regulated by SNHG4/miR-204-5p axis inhibits ferroptosis to aggravate the progression of hepatocellular carcinoma [ Discov Oncol, 2025, 16(1):1349]	PubMed: 40670855
Chinese medicinal formula Fu Xin decoction against chronic heart failure by inhibiting the NLRP3/caspase-1/GSDMD pyroptotic pathway [ Biomed Pharmacother, 2024, 174:116548]	PubMed: 38599064
Pre-treatment with notoginsenoside R1 from Panax notoginseng protects against high-altitude-induced pulmonary edema by inhibiting pyroptosis through the NLRP3/caspase-1/GSDMD pathway [ Biomed Pharmacother, 2024, 180:117512]	PubMed: 39353320

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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