Nile Red

製品コードS6818 バッチS681801

化学情報

	Synonyms	Nile Blue A oxazone, Phenoxazone 9	Storage (From the date of receipt)	3 years -20°C(in the dark) powder
	化学式	C₂₀H₁₈N₂O₂
	分子量	318.37	CAS No.	7385-67-3
Solubility (25°C)*	体外	DMSO	40 mg/mL (125.63 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Nile Red (Nile Blue A oxazone, Phenoxazone 9) is an excellent vital fluorescent stain for the detection of intracellular lipid droplets in the presence of a hydrophobic environment. Nile Red is applied for staining intracellular lipids, hydrophobic domains of proteins and lysosomal phospholipid inclusions.
in vitro	1. Preparation of Phalloidin-TRITC working solution 1.1Preparation of the stock solution Dissolve Phalloidin-TRITC in Methanol to obtain 10 mM of stock solution. Note: It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Phalloidin-TRITC working solution Dilute the stock solution in serum-free cell culture medium to obtain 1-10 μM of working solution. Note: Please adjust the concentration of Phalloidin-TRITC working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells (6-well plate) a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. b.Add 1 mL of working solution, and then incubate at room temperature for 30-60 minutes. c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. d.Wash twice with PBS, 5 minutes each time. e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a.Culture adherent cells on sterile coverslips. b.Remove the coverslip from the medium and aspirate excess medium. c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes. d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

製品説明

Nile Red (Nile Blue A oxazone, Phenoxazone 9) is an excellent vital fluorescent stain for the detection of intracellular lipid droplets in the presence of a hydrophobic environment. Nile Red is applied for staining intracellular lipids, hydrophobic domains of proteins and lysosomal phospholipid inclusions.

in vitro

1. Preparation of Phalloidin-TRITC working solution
1.1Preparation of the stock solution
Dissolve Phalloidin-TRITC in Methanol to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Phalloidin-TRITC working solution
Dilute the stock solution in serum-free cell culture medium to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Phalloidin-TRITC working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
b.Add 1 mL of working solution, and then incubate at room temperature for 30-60 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

プロトコル(参考用のみ)

参考

[1] Greenspan P, et al. J Cell Biol. 1985 Mar;100(3):965-73.

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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