NKL 22

製品コードS6548 バッチS654801

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₉H₂₃N₃O₂
	分子量	325.40	CAS No.	537034-15-4
Solubility (25°C)*	体外	DMSO	65 mg/mL (199.75 mM)
		Ethanol	3 mg/mL (9.21 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	NKL 22 is a selective inhibitor of HDAC with IC50 of 78 µM.
in vitro	2.5 μM of NKL 22 increases frataxin protein concentrations in the FRDA ymphoid cell line. Incubation for 2 d in culture with NKL 22 increases the FXN mRNA level in lymphocytes from the affected individual to about 80% of that in lymphocytes from the unaffected individual. NKL 22 is highly active in increasing FXN mRNA levels in cells obtained from both carrier C and affected AC, bringing the FXN mRNA level in the FRDA lymphocytes to about 160% of that found in the untreated carrier lymphocytes.^[1].

製品説明

NKL 22 is a selective inhibitor of HDAC with IC50 of 78 µM.

in vitro

2.5 μM of NKL 22 increases frataxin protein concentrations in the FRDA ymphoid cell line. Incubation for 2 d in culture with NKL 22 increases the FXN mRNA level in lymphocytes from the affected individual to about 80% of that in lymphocytes from the unaffected individual. NKL 22 is highly active in increasing FXN mRNA levels in cells obtained from both carrier C and affected AC, bringing the FXN mRNA level in the FRDA lymphocytes to about 160% of that found in the untreated carrier lymphocytes.^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	human lymphocytes
	濃度	2.5 μM
	反応時間	48 h
	実験の流れ	Blood is collected in heparinized Vacutainer tubes and lymphocytes are isolated by density centrifugation using Ficoll-Paque PLUS. Lymphocytes are maintained in the same culture medium and conditions as the established cell lines. HDAC inhibitors are dissolved in DMSO and added the solution to the culture medium at the concentrations, for the indicated times. The final DMSO concentration in the culture medium does not exceed 0.5% (v/v). Cells are treated with HDAC inhibitors after 16 h, and RNA is isolated after subsequent 48-h incubation.

参考

[1] Herman D, et al. Nat Chem Biol. 2006 ;2(10):551-8.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。