(−)-Norepinephrine

製品コードS9507 バッチS950701

印刷

化学情報

Chemical Structure Synonyms Arterenol, Levarterenol, L-Noradrenaline Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
1 year -80°C(in the dark) in solvent
化学式

C8H11NO3
分子量 169.18 CAS No. 51-41-2
Solubility (25°C)* 体外 DMSO 11 mg/mL (65.01 mM)
Water Insoluble
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 (−)-Norepinephrine (Arterenol, Levarterenol, L-Noradrenaline) is a natural neurotransmitter and hormone. It is an agonist of adrenergic receptors with Ki values of 330, 56, and 740 nM for α1, α2, and β1 adrenoceptors, respectively.
in vitro

(-)-Norepinephrine inhibits the production of TNF-α, IL-6, and CXCL2/MIP-2 and promotes the secretion of IL-10 from LPS-stimulated murine alveolar macrophages. (-)-Norepinephrine down-regulates NF-κB activation in stimulated alveolar macrophages. [2]
in vivo

Injection of (-)-Norepinephrine into senescence-accelerated prone 8 (SAMP8) mice reverses formaldehyde accumulation and (-)-Norepinephrine deficiency and restored the magnitude of long-term potentiation and memory. [3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 MH-S murine alveolar macrophage cells
濃度 0.01μM, 0.1μM, 1 μM
反応時間
実験の流れ

After plating on to 48-well plates (2×105 cells/well), MH-S cells are stimulated with LPS (10 ng/ml) for 6, 12, 18, or 24 h to select the optimum exposure length. In the second step, cells are incubated with norepinephrine (0, 0.01, 0.1, or 1 μM) for 30min and then incubated with or without LPS (10ng/ml) for 6h. In the third step, cells are pre-incubated with BRL (0, 2, 5, or 10 μM) for 30 min and treated with norepinephrine (1 μM) or PBS for a further 30 min, then stimulated with LPS (10 ng/ml) or maintained in PBS for 6 h. The cell culture supernatant is collected and stored at 80 C for cytokine detection. Total protein is extracted from cells for Western blot analysis. The cytotoxicity of norepinephrine, BRL, and LPS to MH-S cells are measured by CCK-8.
動物実験 動物モデル Senescence-accelerated prone 8 (SAMP8) mice, senescence-resistant 1 (SAMR1) mice
投薬量 10 μM (2 μL)
投与方法 i.c.v.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Generation of self-organized autonomic ganglion organoids from fibroblasts [ iScience, 2023, 26(3):106241] PubMed: 36922996
Suppression of trabecular meshwork phagocytosis by norepinephrine is associated with nocturnal increase in intraocular pressure in mice [ Commun Biol, 2022, 5(1):339] PubMed: 35396348
Inhibition of STAT3 enhances UCP1 expression and mitochondrial function in brown adipocytes [ Eur J Pharmacol, 2022, 926:175040] PubMed: 35598846
α2A-adrenoceptor deficiency attenuates lipopolysaccharide-induced lung injury by increasing norepinephrine levels and inhibiting alveolar macrophage activation in acute respiratory distress syndrome [ Clin Sci (Lond), 2020, 134(14):1957-1971] PubMed: 32643759
Berberine Attenuates Cardiac Hypertrophy Through Inhibition of mTOR Signaling Pathway [ Cardiovasc Drugs Ther, 2020, 11] PubMed: 32394178

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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