NPD8733

製品コードS4453 バッチS445301

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₈H₁₅NO₄
	分子量	309.32	CAS No.	696655-62-6
Solubility (25°C)*	体外	DMSO	31 mg/mL (100.21 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	NPD8733 is an inhibitor of cancer cell-enhanced fibroblast migration that specifically binds to valosin-containing protein (VCP)/p97, a member of the ATPase-associated with diverse cellular activities (AAA+) protein family.
in vitro	NPD8733 is identified as inhibiting the enhanced migration of fibroblasts when co-cultured with cancer cells, in which valosin-containing protein (VCP)/p97 is the target protein for NPD8733 .^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	NIH3T3 cells and MCF7 cells
	濃度	30 μl
	反応時間	3 h
	実験の流れ	NPD8733 beads were prepared. The co-cultured NIH3T3 cells and MCF7 cells (5:1; 2 × 10⁵ and 0.4 × 10⁵, respectively) were collected, washed with PBS, and resuspended in binding buffer and a protease inhibitor mixture tablet. After cells were lysed by homogenization with sonication, the insoluble material was discarded by centrifugation, and the supernatant was collected as the cell lysate. Eight milligrams of protein from the cell lysate was incubated with 30 μl of control, NPD8733 beads for 3 h at 4 °C using a rotating mixer.

参考

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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