NSC348884

製品コードS8149 バッチS814902

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₃₈H₄₀N₁₀

分子量

636.79

CAS No.

81624-55-7

Solubility (25°C)*

体外

DMSO

100 mg/mL (157.03 mM)

Ethanol

100 mg/mL (157.03 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.500mg/ml (3.93mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.250mg/ml (1.96mM)	Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	NSC348884は、nucleophosmin阻害剤として、さまざまな癌細胞株において1.4～4 µMのIC50値で細胞増殖を阻害し、アポトーシスを誘導します。
in vitro	NSC348884 is a putative nucleophosmin small molecular inhibitor that disrupts a defined hydrophobic pocket required for oligomerization. It disrupts nucleophosmin oligomer formation by native polyacrylamide gel electrophoresis assay. This compound upregulates p53 and induces apoptosis.
in vivo	In vivo invasion and intravasation (that is, the number of CTCs) are significantly inhibited after injection of NSC348884 (as an inhibitor of NPM1 oligomerization) into the tumor-bearing mice. No significant difference in overall cell death is observed by histology in the treated tumors with the 4-hour brief treatments, suggesting that the inhibition seen is specific to migration..

プロトコル(参考用のみ)

細胞アッセイ	細胞株	LNCaP cells/Granta cells
	濃度	0.01–60 μM for LNCaP cells; 0.5–2.8 μM for Granta cells
	反応時間
	実験の流れ
動物実験	動物モデル	Mice bearing either orthotopic MDA-MB-231-GFP tumors (MDA231) or patient-derived HT17 and HT19 tumors
	投薬量	25 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/18345031/
https://pubmed.ncbi.nlm.nih.gov/23113900/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The nucleolar granular component mediates genome-nucleolus interactions and establishes their repressive chromatin states [ Mol Cell, 2025, S1097-2765(25)00409-5]	PubMed: 40412390
The EGFR/ErbB inhibitor neratinib modifies the neutrophil phosphoproteome and promotes apoptosis and clearance by airway macrophages [ Front Immunol, 2022, 13:956991]	PubMed: 35967296
NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer. [ Nat Commun, 2020, 3;11(1):1669]	PubMed: 32245950
Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid [ Virulence, 2020, 11(1):1379-1393]	PubMed: 33073687
Nucleolar stress regulates stromal-epithelial transition via NPM1 during decidualization [ Reproduction, 2020, 160(4):491-500]	PubMed: 32817586
Nucleolar stress regulation of endometrial receptivity in mouse models and human cell lines. [ Cell Death Dis, 2019, 10(11):831]	PubMed: 31685803

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。