Datasheet

生物学的記述

Specificity	NTF2 Antibody [A24B24] detects endogenous levels of total NTF2 protein.
Background	NTF2 (nuclear transport factor 2), a highly conserved cytosolic homodimer, serves as the dedicated import receptor for RanGDP, the master regulator of all nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope reformation. It features a stable β-barrel structure that forms a deep hydrophobic pocket selectively recognizing the GDP-bound conformation of Ran through critical contacts at switch I/II loops and the C-terminal tail. The core mechanism hinges on NTF2 binding RanGDP with high affinity in the cytoplasm, facilitating its translocation through nuclear pore complexes via interactions with nucleoporins like p62 and Nup50, where RCC1 rapidly exchanges GDP for GTP to establish the essential RanGTP gradient that powers importin-mediated cargo release and exportin loading. This Ran cycle couples directly to cell cycle progression: NTF2-mediated Ran nuclear accumulation prevents G2/M arrest by suppressing the MAD2 spindle assembly checkpoint, ensuring proper kinetochore-microtubule attachments, while post-mitotic NTF2 activity supports NE reassembly by concentrating Ran around chromatin for importin exclusion. NTF2 sustains protein homeostasis across proliferating tissues, making it indispensable for researchers modeling transport defects in temperature-sensitive yeast mutants or quantifying Ran flux via microinjection assays in mammalian cells. Depletion triggers catastrophic nuclear import failure and mitotic catastrophe, with dysregulation implicated in neurodegenerative proteinopathies from aggregate sequestration.

Specificity

NTF2 Antibody [A24B24] detects endogenous levels of total NTF2 protein.

Background

NTF2 (nuclear transport factor 2), a highly conserved cytosolic homodimer, serves as the dedicated import receptor for RanGDP, the master regulator of all nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope reformation. It features a stable β-barrel structure that forms a deep hydrophobic pocket selectively recognizing the GDP-bound conformation of Ran through critical contacts at switch I/II loops and the C-terminal tail. The core mechanism hinges on NTF2 binding RanGDP with high affinity in the cytoplasm, facilitating its translocation through nuclear pore complexes via interactions with nucleoporins like p62 and Nup50, where RCC1 rapidly exchanges GDP for GTP to establish the essential RanGTP gradient that powers importin-mediated cargo release and exportin loading. This Ran cycle couples directly to cell cycle progression: NTF2-mediated Ran nuclear accumulation prevents G2/M arrest by suppressing the MAD2 spindle assembly checkpoint, ensuring proper kinetochore-microtubule attachments, while post-mitotic NTF2 activity supports NE reassembly by concentrating Ran around chromatin for importin exclusion. NTF2 sustains protein homeostasis across proliferating tissues, making it indispensable for researchers modeling transport defects in temperature-sensitive yeast mutants or quantifying Ran flux via microinjection assays in mammalian cells. Depletion triggers catastrophic nuclear import failure and mitotic catastrophe, with dysregulation implicated in neurodegenerative proteinopathies from aggregate sequestration.

使用情報

Application

WB, IHC, ELISA

Dilution

WB	IHC
1:500-1:2000	1:250-1:1000

Reactivity

Human

Source

Mouse Monoclonal Antibody

MW

14 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/10930458/
https://pubmed.ncbi.nlm.nih.gov/10679025/

Application Data

WB

Validated by Selleck

Lane 1: Hela, Lane 2: HepG2