NVP 231

製品コードS6501 バッチS650102

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₂₅H₂₅N₃O₂S
	分子量	431.55	CAS No.	362003-83-6
Solubility (25°C)*	体外	DMSO	85 mg/mL (196.96 mM)
		Ethanol	4 mg/mL (9.26 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	NVP 231 is a novel CerK inhibitor that inhibits the catalytic activity of recombinant CerK in vitro with an IC50 of 12 nM.
in vitro	NVP-231 reduces cell viability and DNA synthesis by triggering cell death of cancer cells. NVP-231-treated cells committed M phase arrest rather than arrest at the G2/M boundary. In MCF-7 and NCI-H358 cells, NVP-231 treatment causes a concentration-dependent up-regulation of cyclin B1 phosphorylation at Ser133 and a reduction of CDK1 phosphorylation at Tyr15. Wee1 is down-regulated by NVP-231 in both NCI-H358 and MCF-7 cells. Upon NVP-231 treatment of cancer cells, CDK4 protein is concentration- and time-dependently down-regulated. This effect is even more pronounced in synchronized cells^[2].

製品説明

NVP 231 is a novel CerK inhibitor that inhibits the catalytic activity of recombinant CerK in vitro with an IC50 of 12 nM.

in vitro

NVP-231 reduces cell viability and DNA synthesis by triggering cell death of cancer cells. NVP-231-treated cells committed M phase arrest rather than arrest at the G2/M boundary. In MCF-7 and NCI-H358 cells, NVP-231 treatment causes a concentration-dependent up-regulation of cyclin B1 phosphorylation at Ser133 and a reduction of CDK1 phosphorylation at Tyr15. Wee1 is down-regulated by NVP-231 in both NCI-H358 and MCF-7 cells. Upon NVP-231 treatment of cancer cells, CDK4 protein is concentration- and time-dependently down-regulated. This effect is even more pronounced in synchronized cells^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	MCF-7 and NCI H358 cells
	濃度	10, 100, 300, 500 and 1000 nM
	反応時間	4 h
	実験の流れ	MCF-7 and NCI-H358 cells are plated in a 96-well black plate (1 × 10⁴ cells per well) and treated for 48 h with either vehicle (Co) or the indicated concentrations of NVP-231 (in nM). For the last 4 h of treatment, alamarBlueR is added and fluorescence is determined.

参考

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。