O4I1

製品コードS7943 バッチS794301

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C16H15NO2
分子量 253.30 CAS No. 175135-47-4
Solubility (25°C)* 体外 DMSO 51 mg/mL (201.34 mM)
Ethanol (warmed with 50ºC water bath) 1 mg/mL (3.94 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 O4I1 is a potent Oct3/4 inducer.
in vitro O4I1 enhances Oct3/4 gene expression by approximately 2.5- and 4-fold at 10 μM and 20 μM, respectively, increases Oct3/4 protein levels, and promotes Oct3/4 mediated transcriptional activation. [1]

プロトコル(参考用のみ)

キナーゼアッセイ Luciferase Reporter Assay
The luciferase reporter assay is performed using a Beatle juice kit according to the manufacturer’s instructions. Briefly, HEK293-Oct3/4 or NCCIT-Oct3/4 reporter cells are plated into 24-well tissue culture plates at a density of 100,000 per well for 24 h. The cells are treated with compounds as indicated in the text and harvested with 90 μL of Beatle lysis-juice at 37 °C for 15 min. The protein concentration is determined by the Bradford assay. One hundred microliters of reaction mixture containing luciferin and ATP is added to 20 μL of cell lysis substance in a white plate, incubated for 5 min, and measured by a luminometer plate reader. The activity is determined as percent luminous intensity of treated cells over control cells from at least five-independent experiments. The online luciferase reporter assay in living cells is performed by directly adding luciferin to the culture medium as reported.

参考

  • https://pubmed.ncbi.nlm.nih.gov/25898186/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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