O4I2

製品コードS7944 バッチS794401

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C12H11ClN2O2S
分子量 282.75 CAS No. 165682-93-9
Solubility (25°C)* 体外 DMSO 56 mg/mL (198.05 mM)
Ethanol 20 mg/mL (70.73 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 O4I2 is a potent Oct3/4 inducer, which is suitable for iPSC generation.
in vitro O4I2 activates Oct3/4 expression in HEK293 and embryonal NCCIT cells, and also in Oct-deficient HeLa and terminally differentiated human fibroblasts, and also induces other pluripotency-associated genes such as Lin28 and Nanog. [1]

プロトコル(参考用のみ)

キナーゼアッセイ Luciferase Reporter Assay
Beetle juice kit is used to determine the luciferase activity. Briefly, HEK293-Oct3/4 or NCCIT-Oct3/4 reporter cells are seeded into 24-well tissue culture plates at a density of 100000 per well for 24 h. After treatment with compounds as indicated in the text, the cells are harvested with 80 μL of Beetle lysis juice at 37 °C for 10 min. The protein concentration is determined by Bradford assay. Then 100 μL of reaction mixture containing luciferin and ATP is added to 20 μL of cell lysis in a white plate, incubated for 3 min, and measured by a luminometer plate reader. The activity is determined as percent luminous intensity of treated cells over control cells from at least five independent experiments.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Effects of omega-3 polyunsaturated fatty acids on cellular development in human ovarian granulosa tumor cells (KGN) [ Front Nutr, 2022, 9:1017072] PubMed: 36245495
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7] PubMed: 34997030

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。