OAC1

製品コードS7217 バッチS721701

印刷

化学情報

Synonyms

BAS 00287861

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₁₄H₁₁N₃O

分子量

237.26

CAS No.

300586-90-7

Solubility (25°C)*

体外

DMSO

47 mg/mL (198.09 mM)

Ethanol

21 mg/mL (88.51 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.35mg/ml (9.90mM)	Taking the 1 mL working solution as an example, add 50 μL of 47 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.235mg/ml (0.99mM)	Taking the 1 mL working solution as an example, add 50 μL of 4.7 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	OAC1 (Oct4-activating compound 1, BAS 00287861)は、再プログラミングの効率を高めることができます。
in vitro	OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, this compound enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using it along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. It seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. This chemical may be used to enhance the reprogramming of somatic cells to a pluripotent state.

製品説明

OAC1 (Oct4-activating compound 1, BAS 00287861)は、再プログラミングの効率を高めることができます。

in vitro

OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, this compound enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using it along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. It seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. This chemical may be used to enhance the reprogramming of somatic cells to a pluripotent state.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human ESCs transfected with human Oct4 and Nanog luciferase reporter genes
	濃度	~1 μM
	反応時間	24 h
	実験の流れ	The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after this compound treatment.

参考

https://pubmed.ncbi.nlm.nih.gov/23213213/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A stem cell zoo uncovers intracellular scaling of developmental tempo across mammals [ Cell Stem Cell, 2023, 30(7):938-949.e7]	PubMed: 37343565
Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification [ J Vis Exp, 2023, (193).]	PubMed: 37036224
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7]	PubMed: 34997030

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。