OAC1

製品コードS7217 バッチS721701

化学情報

	Synonyms	BAS 00287861	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₄H₁₁N₃O
	分子量	237.26	CAS No.	300586-90-7
Solubility (25°C)*	体外	DMSO	47 mg/mL (198.09 mM)
		Ethanol	21 mg/mL (88.51 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	OAC1 (Oct4-activating compound 1, BAS 00287861) can enhance the efficiency of reprogramming.
in vitro	OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, OAC1 enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using OAC1 along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. OAC1 may be used to enhance the reprogramming of somatic cells to a pluripotent state. ^[1]

製品説明

OAC1 (Oct4-activating compound 1, BAS 00287861) can enhance the efficiency of reprogramming.

in vitro

OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, OAC1 enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using OAC1 along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. OAC1 may be used to enhance the reprogramming of somatic cells to a pluripotent state. ^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human ESCs transfected with human Oct4 and Nanog luciferase reporter genes
	濃度	~1 μM
	反応時間	24 h
	実験の流れ	The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after OAC1 treatment.

参考

[1] Li W, et al. Proc Natl Acad Sci U S A, 2012, 109(51), 20853-20858.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A stem cell zoo uncovers intracellular scaling of developmental tempo across mammals [ Cell Stem Cell, 2023, 30(7):938-949.e7]	PubMed: 37343565
Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification [ J Vis Exp, 2023, (193).]	PubMed: 37036224
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7]	PubMed: 34997030

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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