Oleic Acid

製品コードS4707 バッチS470703

印刷

化学情報

Chemical Structure Synonyms Oleate, 9-Octadecenoic Acid, Elaidoic acid Storage
(From the date of receipt)		 2 years  -20°C  liquid
化学式

C18H34O2
分子量 282.46 CAS No. 112-80-1
Solubility (25°C)* 体外 Ethanol 100 mg/mL (354.03 mM)
DMSO 50 mg/mL (177.01 mM)
 
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 2.500mg/ml (8.85mM) Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 2.500mg/ml (8.85mM) Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Oleic acid (Oleate, 9-Octadecenoic Acid, Elaidoic acid) is a fatty acid that occurs naturally in various animal and vegetable fats and oils. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. Oleic Acid can be used to induce animal models of Renal Failure.
in vitro Oleic acid treatment induces a marked increase in the proliferation of 786-O cells in a dose-dependent manner. It delays 786-O cell apoptosis. In 786‑O cells, ILK is upregulated by oleic acid treatment. Oleic acid activates G protein-coupled receptors and phosphorylates ERK1/2 to induce cancer cell proliferation in breast cancer[1]. Oleic acid(as low as 5 M) inhibits the stearic acid-induced increase in intercellular adhesion molecule-1 (ICAM-1) expression. Oleic acid is able to diminish endothelial cell activation, irrespective of the stimulus utilized, by suppressing the upregulation of adhesion molecules. It can protect Chinese hamster ovary cells from palmitate-induced lipotoxicity by promoting triglyceride synthesis within the cells[2].
in vivo Oleic acid inhibits in vivo Na/K-ATPase activity in the lungs and induced lung injury. 10 μM oleic acid induces lung edema and inflammation with cell influx, lipid body formation, and leukotriene B4 (LTB4) and prostaglandin E2 (PGE2) production[3]. Oleic acid ameliorates amyloidosis in mouse models of Alzheimer's disease[4].
密度 0.89 g/mL at 25 °C

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human RCC（renal cell carcinoma） cell line, 786-O.
濃度 0.05, 0.1, 0.2 mmol/l
反応時間 48 h
実験の流れ

Cells (2x103 cells/well) are seeded in 96-well microtitre plates and incubated for 24 h in 100 µl culture medium. Cells in the experimental group were then treated with 0.05, 0.1 or 0.2 mmol/l oleic acid for 48 h. MTT [20 µl (5 g/l)] is added to the cells which were then cultivated for a further 4 h. Following the removal of the supernatant fluid, 150 µl/well DMSO is added to the cells which are agitated for 15 min. Absorbance is measured at 490 nm by an ELISA reader.
動物実験 動物モデル male Swiss mice
投薬量 2.5, 5.0 or 10.0 μmol
投与方法 i.v.

参考

  • https://pubmed.ncbi.nlm.nih.gov/23599801/
  • https://pubmed.ncbi.nlm.nih.gov/20852092/
  • https://pubmed.ncbi.nlm.nih.gov/24175969/
  • https://pubmed.ncbi.nlm.nih.gov/21040071/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

GSK621 ameliorates lipid accumulation via AMPK pathways and reduces oxidative stress in hepatocytes in vitro and in obese mice in vivo [ Life Sci, 2025, 374:123687] PubMed: 40334907
Ginkgolide B attenuates hyperlipidemia by restoring sphingolipid homeostasis and activating PPARα and Nrf2 pathways [ Sci Rep, 2025, 15(1):28774] PubMed: 40770408
Oleic acid activates TGFβ-Smad3 signaling to promote ovarian cancer progression [ J Ovarian Res, 2025, 18(1):180] PubMed: 40790593
PROX1 drives neuroendocrine plasticity and liver metastases in prostate cancer [ Cancer Lett, 2024, 597:217068] PubMed: 38901665
Sodium Orthovanadate Mitigates Nonalcoholic Fatty Liver Disease by Enhancing Autophagy [ Biol Pharm Bull, 2024, 47(10):1786-1795] PubMed: 39477507
Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response [ Cell Rep Med, 2023, 4(10):101234] PubMed: 37852179
SLC25A22 as a Key Mitochondrial Transporter Against Ferroptosis by Producing Glutathione and Monounsaturated Fatty Acids [ Antioxid Redox Signal, 2023, 39(1-3):166-185.] PubMed: 37051693
SIRT1 prevents cigarette smoking-induced lung fibroblasts activation by regulating mitochondrial oxidative stress and lipid metabolism [ J Transl Med, 2022, 20(1):222] PubMed: 35568871
FGF9 Alleviates the Fatty Liver Phenotype by Regulating Hepatic Lipid Metabolism [ Front Pharmacol, 2022, 13:850128] PubMed: 35517790
MEN1 promotes ferroptosis by inhibiting mTOR-SCD1 axis in pancreatic neuroendocrine tumors [ Acta Biochim Biophys Sin (Shanghai), 2022, 54(11):1599-1609] PubMed: 36604142

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。