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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C26H21FN4O |
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分子量 | 424.47 | CAS No. | 1800487-55-1 | |
Solubility (25°C)* | 体外 | DMSO | 85 mg/mL (200.24 mM) | |
Ethanol | 85 mg/mL (200.24 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | OT-82 is a novel nicotinamide phosphoribosyltransferase (NAMPT) inhibitor with average IC50s of 13.03 nM in non-HP cancer cells and 2.89 nM in HP cancer cells, respectively. |
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in vitro | OT-82 is an effective nicotinamide phosphoribosyltransferase (NAMPT) inhibitor, thus inhibits the salvage pathway of NAD synthesis to kill the neoplastic cells of hematopoietic. The average IC50 is 13.03 nM in non-HP cancer cells and 2.89 nM in HP cancer cells.<sup><a class="sref" href="#s_ref">[1]</a></sup> |
in vivo | OT-82 has therapeutic effect in subcutaneous(SC) and systemic mouse xenograft models of HP malignancies.<sup><a class="sref" href="#s_ref">[1]</a></sup> |
細胞アッセイ | 細胞株 | MV4–11 cells |
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濃度 | -- | |
反応時間 | 1 h | |
実験の流れ | MV4 11 cell extract (3 mg protein/reaction) was incubated for 1 h at 40 ℃ with Affi-Gel 10 coupled with "active" compound (OT-82), "inactive" compound (a non-cytotoxic OT- 82 structural analog), or a combination of the two ("competition"). Eluted proteins were subjected to gel electrophoresis and silver staining or transferred to nitrocellulose membrane for western blotting with NAMPT antisera. |
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動物実験 | 動物モデル | Ubcutaneous (SC) and systemic mouse xenograft models of HP malignancies |
投薬量 | 25 or 50 mg/kg for 6 consecutive days per week for 3 weeks | |
投与方法 | p.o. |
Inhibition of nicotinamide dinucleotide salvage pathway counters acquired and intrinsic poly(ADP-ribose) polymerase inhibitor resistance in high-grade serous ovarian cancer [ Sci Rep, 2023, 13(1):3334] | PubMed: 36849518 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
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