Datasheet

生物学的記述

Specificity	PARK7/DJ1 Antibody [D23K10] detects endogenous levels of total PARK7/DJ1 protein.
Background	PARK7/DJ-1, a ubiquitously expressed dimeric protein with a conserved α-helical fold featuring a flexible nucleophile-sensing cysteine, functions primarily as an oxidative stress sentinel that maintains redox homeostasis, mitochondrial integrity, and protein quality control across neurons and peripheral tissues. Upon reactive oxygen species accumulation, DJ-1 undergoes selective oxidation at Cys106 to form a stable sulfinate that enhances dimer stability and catalytic reactivity, enabling it to directly scavenge H2O2 while allosterically activating Nrf2 transcription by disrupting Keap1 sequestration; this unleashes antioxidant response element-driven genes encoding SOD1/2, GPx, and NQO1 for cytoprotection. Concurrently, oxidized DJ-1 stabilizes the Trx1-ASK1 inhibitory complex to block MAPK/JNK-mediated apoptosis while promoting PI3K/Akt signaling through PTEN oxidation, fostering cell survival and dopaminergic neuron resilience under mitochondrial stress. DJ-1 translocates to mitochondria under paraquat or rotenone exposure to chaperone α-synuclein aggregation and facilitate mitophagy via Parkin recruitment, while nuclear accumulation coactivates androgen receptor and p53-dependent transcription for neuroprotection and DNA repair. DJ-1 sustains dopaminergic function in substantia nigra through D2 receptor trafficking and long-term depression while protecting erythrocytes from oxidant-induced hemolysis during erythropoiesis. Loss-of-function mutations cause early-onset recessive Parkinson's through failed Nrf2 induction and α-synucleinopathy, while peripheral neuropathy arises from impaired sensory neuron cold tolerance.

Specificity

PARK7/DJ1 Antibody [D23K10] detects endogenous levels of total PARK7/DJ1 protein.

Background

PARK7/DJ-1, a ubiquitously expressed dimeric protein with a conserved α-helical fold featuring a flexible nucleophile-sensing cysteine, functions primarily as an oxidative stress sentinel that maintains redox homeostasis, mitochondrial integrity, and protein quality control across neurons and peripheral tissues. Upon reactive oxygen species accumulation, DJ-1 undergoes selective oxidation at Cys106 to form a stable sulfinate that enhances dimer stability and catalytic reactivity, enabling it to directly scavenge H2O2 while allosterically activating Nrf2 transcription by disrupting Keap1 sequestration; this unleashes antioxidant response element-driven genes encoding SOD1/2, GPx, and NQO1 for cytoprotection. Concurrently, oxidized DJ-1 stabilizes the Trx1-ASK1 inhibitory complex to block MAPK/JNK-mediated apoptosis while promoting PI3K/Akt signaling through PTEN oxidation, fostering cell survival and dopaminergic neuron resilience under mitochondrial stress. DJ-1 translocates to mitochondria under paraquat or rotenone exposure to chaperone α-synuclein aggregation and facilitate mitophagy via Parkin recruitment, while nuclear accumulation coactivates androgen receptor and p53-dependent transcription for neuroprotection and DNA repair. DJ-1 sustains dopaminergic function in substantia nigra through D2 receptor trafficking and long-term depression while protecting erythrocytes from oxidant-induced hemolysis during erythropoiesis. Loss-of-function mutations cause early-onset recessive Parkinson's through failed Nrf2 induction and α-synucleinopathy, while peripheral neuropathy arises from impaired sensory neuron cold tolerance.

使用情報

Application

WB, IP, IF

Dilution

WB	IP	IF
1:1000	1:100	1:100

Reactivity

Human, Mouse, Rat, Hamster, Monkey

Source

Rabbit Monoclonal Antibody

MW

22 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/39486088/
https://pubmed.ncbi.nlm.nih.gov/32612601/

Application Data

WB

Validated by Selleck

Lane 1: MEF, Lane 2: MEF (KO DJ-1), Lane 3: Hela, Lane 4: C6