受注:045-509-1970 |
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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C11H8FNO2S
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分子量 | 237.25 | CAS No. | 179461-52-0 | |
Solubility (25°C)* | 体外 | DMSO | 47 mg/mL (198.1 mM) | |
Ethanol | 47 mg/mL (198.1 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | PD 151746 is a selective, cell-permeable calpain inhibitor with Ki of 0.26 μM for μ-Calpain, about 20-fold selectivity over m-calpain. |
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in vitro | In SY5Y cells, PD151746 effectively attenuates the SLLVY-AMC hydrolysis induced by maitotoxin. [1] In HMEC-1 cells, PD 151746 decreases cytotoxicity induced by oxidized low-density lipoprotein (oxLDL). [2] |
キナーゼアッセイ | Protease Assays | |
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The calpain microplate assay is carried out as followed. Briefly, a mixture containing 0.5 mg/ml casein, 20 mM DTT, 50 mM Tris-HCl (pH 7.4), 0.01 unit purified, μ-calpain (human erythrocytes) or m-calpain (rabbit skeletal muscle) (about 0.08-0.12 ,ug) in the presence of various concentrations of an inhibitor. CaCl2 (4 mM) is added to a microtiter plate (250 ,tl) and incubated for 60 min at 25癈. The samples are then processed for colorimetric development and read on a Molecular Devices Thermomax microplate reader at an absorbance of 595 nm. Apparent Ki values are calculated. |
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Data from [Data independently produced by , , PLoS One, 2017, 12(1):e0169788. ]
Hepatitis C virus induces oxidation and degradation of apolipoprotein B to enhance lipid accumulation and promote viral production [ PLoS Pathog, 2021, 17(9):e1009889] | PubMed: 34492079 |
Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke [ Cell, 2019, 177(5):1262-1279] | PubMed: 31056284 |
Myocardial caspase-3 and NF-κB activation promotes calpain-induced septic apoptosis: The role of Akt/eNOS/NO pathway. [ Life Sci, 2019, 222:195-202] | PubMed: 30807754 |
Polychlorinated biphenyl quinone-induced signaling transition from autophagy to apoptosis is regulated by HMGB1 and p53 in human hepatoma HepG2 cells. [ Toxicol Lett, 2019, 306:25-34] | PubMed: 30742880 |
Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in Psoriasis. [ PLoS One, 2017, 12(1):e0169788] | PubMed: 28060905 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。