PDD00017273

製品コードS8862 バッチS886202

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₃H₂₆N₆O₄S₂

分子量

514.62

CAS No.

1945950-21-9

Solubility (25°C)*

体外

DMSO

50 mg/mL (97.15 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O

5.0mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	PDD00017273 is a potent and selective poly (ADP ribose) glycohydrolase (PARG) inhibitor with an IC50 of 26 nM. PDD00017273 exhibits >350-fold selectivity for PARG over a panel of ion channels, enzymes and receptors, including PARP1 and ARH3.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Hela cells
	濃度	0.01-30 μM
	反応時間	72 h
	実験の流れ	HeLa cells are seeded in 30 μL media at 1×10⁴ cells/mL in 384-well plates. 16-24 h later, cells are treated with inhibitors (8 pt dose response, 0.01-30 μM, triplicates) or vehicle (DMSO) control. The outer wells are left un-dosed to account for edge effects. After 72 h, 50 μL of 3.7% Formaldehyde/PBS is added to each well and cells are fixed for 20 min. Cells are then rinsed twice with PBS and stained for 1 h with Hoechst 33342/PBS (1:2000) in the dark. After two further rinses with PBS, images are captured and nuclei counted on a CellInsight.

参考

[1] James DI, et al. ACS Chem Biol. 2016, 11(11):3179-3190.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The three-dimensional structure of the EBV genome plays a crucial role in regulating viral gene expression in EBVaGC [ Nucleic Acids Res, 2023, 10.1093/nar/gkad936]	PubMed: 37889078
The regulation loop of MARVELD1 interacting with PARP1 in DNA damage response maintains genome stability and promotes therapy resistance of cancer cells [ Cell Death Differ, 2023, 10.1038/s41418-023-01118-z]	PubMed: 36750717
Fatty acid oxidation facilitates DNA double-strand break repair by promoting PARP1 acetylation [ Cell Death Dis, 2023, 14(7):435]	PubMed: 37454129
Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications [ J Vis Exp, 2023, (194).]	PubMed: 37154555
PARP1 Inhibition Halts EBV+ Lymphoma Progression by Disrupting the EBNA2/MYC Axis [ bioRxiv, 2023, 2023.07.05.547847]	PubMed: 37461649
The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activity [ Nat Commun, 2022, 13(1):187]	PubMed: 35039491
Micropeptide PACMP inhibition elicits synthetic lethal effects by decreasing CtIP and poly(ADP-ribosyl)ation [ Mol Cell, 2022, S1097-2765(22)00085-5]	PubMed: 35219381
PARP1 and CHK1 coordinate PLK1 enzymatic activity during the DNA damage response to promote homologous recombination-mediated repair [ Nucleic Acids Res, 2021, gkab584]	PubMed: 34197606
PARP1 blockade is synthetically lethal in XRCC1 deficient sporadic epithelial ovarian cancers [ Cancer Lett, 2020, 469:124-133]	PubMed: 31669203
PARP1 blockade is synthetically lethal in XRCC1 deficient sporadic epithelial ovarian cancers. [ Cancer Lett, 2020, 469:124-133]	PubMed: 31669203

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。